Antibodies to cerebellar nerve fibres in two patients with paraneoplastic cerebellar ataxia

Sabrina Balegno, Mauro Ceroni, Manuel Corato, Diego Franciotta, Bruno Giometto, Kalliope Marinu-Aktipu, Rosanna Nano

Research output: Contribution to journalArticle

Abstract

The aim of this study was to characterize two new atypical anti-neuronal antibodies using an immunohistochemical method on rat cerebellum and Western blot techniques with primate cerebellar tissue and with recombinant neuronal proteins. Atypical sera from two patients with paraneoplastic neurological syndromes associated with different tumours were detected. Case number 1 presented cerebellar degeneration and Merkel cell carcinoma and case number 2 paraneoplastic brainstem encephalitis and malignant fibrous histiocytoma. By immunohistochemistry, the two new atypical antibodies showed a similar fibrillar positivity in the molecular and granular layers and around the Purkinje cells. The dot blot with recombinant neuronal proteins (HuD, NOVA-1, CDR62/Yo, Amphiphysin) was negative, whereas the Western blot with neuronal antigens of primate cerebellum identified two different proteins with molecular weights (64 kD in case number 1, and 70 kD in case number 2). In conclusion, the two new antibody reactivities against nerve fibres should be integrated into the diagnostic paraneoplastic neurological syndromes guidelines.

Original languageEnglish
Pages (from-to)3211-3214
Number of pages4
JournalAnticancer Research
Volume25
Issue number5
Publication statusPublished - Sep 2005

Keywords

  • Atypical antibodies
  • Paraneoplastic neurological syndromes
  • Rat cerebellum
  • Western blot

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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