Antibodies to synaptophysin interfere with transmitter secretion at neuromuscular synapses

Janet Alder, Zuo Ping Xie, Flavia Valtorta, Paul Greengard, Mu ming Poo

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

The involvement of synaptophysin, a synaptic vesicle-specific protein, in transmitter release at neuromuscular synapses was studied by intracellular application of synaptophysin antibodies into presynaptic neurons. Polyclonal antibodies or their Fab fragments were loaded into spinal neurons by injection into one of the early blastomeres of Xenopus embryos 1 day prior to culturing or, alternatively, directly through a whole-cell recording pipette at the soma of cultured neurons. At synapses made by antibody-loaded neurons in culture, the spontaneous synaptic currents showed marked reduction in frequency without significant change in their mean amplitude. The impulse-evoked synaptic currents showed reduced amplitude and increased failure rate. These results suggest that interference with synaptophysin function by antibody binding inhibits transmitter secretion.

Original languageEnglish
Pages (from-to)759-768
Number of pages10
JournalNeuron
Volume9
Issue number4
DOIs
Publication statusPublished - 1992

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Synaptophysin
Synapses
Neurons
Antibodies
Spinal Injections
Blastomeres
Immunoglobulin Fab Fragments
Synaptic Vesicles
Carisoprodol
Patch-Clamp Techniques
Xenopus
Embryonic Structures
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Antibodies to synaptophysin interfere with transmitter secretion at neuromuscular synapses. / Alder, Janet; Xie, Zuo Ping; Valtorta, Flavia; Greengard, Paul; Poo, Mu ming.

In: Neuron, Vol. 9, No. 4, 1992, p. 759-768.

Research output: Contribution to journalArticle

Alder, Janet ; Xie, Zuo Ping ; Valtorta, Flavia ; Greengard, Paul ; Poo, Mu ming. / Antibodies to synaptophysin interfere with transmitter secretion at neuromuscular synapses. In: Neuron. 1992 ; Vol. 9, No. 4. pp. 759-768.
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