Antibody-feeding assay

A method to track the internalization of neuropilin-1 and other cell surface receptors

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Ligand-induced endocytosis of receptors exposed on the plasma membrane is a fundamental regulatory step for their functional activation and interaction with intracellular signal transducers. Thus, elucidating the timing of endocytosis and tracing the intracellular fate of receptors is instrumental to understand their signaling cascade in different conditions. Here we describe an assay for the study of endocytosis and intracellular trafficking of individual surface receptors, in living cells subject to different experimental challenges. We applied this method for studying the functional interaction between semaphorin coreceptor Neuropilin-1 and a tyrosine kinase receptor exposed on the cell surface.

Original languageEnglish
Pages (from-to)311-319
Number of pages9
JournalMethods in Molecular Biology
Volume1493
DOIs
Publication statusPublished - 2017

Fingerprint

Neuropilin-1
Cell Surface Receptors
Endocytosis
Antibodies
Semaphorins
Receptor Protein-Tyrosine Kinases
Transducers
Cell Membrane
Ligands

Keywords

  • Immunofluorescence
  • Neuropilin
  • Receptor endocytosis
  • Semaphorin
  • Signaling

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

@article{373ff92947a84e79a2b9e9bf86bef52d,
title = "Antibody-feeding assay: A method to track the internalization of neuropilin-1 and other cell surface receptors",
abstract = "Ligand-induced endocytosis of receptors exposed on the plasma membrane is a fundamental regulatory step for their functional activation and interaction with intracellular signal transducers. Thus, elucidating the timing of endocytosis and tracing the intracellular fate of receptors is instrumental to understand their signaling cascade in different conditions. Here we describe an assay for the study of endocytosis and intracellular trafficking of individual surface receptors, in living cells subject to different experimental challenges. We applied this method for studying the functional interaction between semaphorin coreceptor Neuropilin-1 and a tyrosine kinase receptor exposed on the cell surface.",
keywords = "Immunofluorescence, Neuropilin, Receptor endocytosis, Semaphorin, Signaling",
author = "Sabrina Rizzolio and Luca Tamagnone",
year = "2017",
doi = "10.1007/978-1-4939-6448-2_23",
language = "English",
volume = "1493",
pages = "311--319",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

TY - JOUR

T1 - Antibody-feeding assay

T2 - A method to track the internalization of neuropilin-1 and other cell surface receptors

AU - Rizzolio, Sabrina

AU - Tamagnone, Luca

PY - 2017

Y1 - 2017

N2 - Ligand-induced endocytosis of receptors exposed on the plasma membrane is a fundamental regulatory step for their functional activation and interaction with intracellular signal transducers. Thus, elucidating the timing of endocytosis and tracing the intracellular fate of receptors is instrumental to understand their signaling cascade in different conditions. Here we describe an assay for the study of endocytosis and intracellular trafficking of individual surface receptors, in living cells subject to different experimental challenges. We applied this method for studying the functional interaction between semaphorin coreceptor Neuropilin-1 and a tyrosine kinase receptor exposed on the cell surface.

AB - Ligand-induced endocytosis of receptors exposed on the plasma membrane is a fundamental regulatory step for their functional activation and interaction with intracellular signal transducers. Thus, elucidating the timing of endocytosis and tracing the intracellular fate of receptors is instrumental to understand their signaling cascade in different conditions. Here we describe an assay for the study of endocytosis and intracellular trafficking of individual surface receptors, in living cells subject to different experimental challenges. We applied this method for studying the functional interaction between semaphorin coreceptor Neuropilin-1 and a tyrosine kinase receptor exposed on the cell surface.

KW - Immunofluorescence

KW - Neuropilin

KW - Receptor endocytosis

KW - Semaphorin

KW - Signaling

UR - http://www.scopus.com/inward/record.url?scp=84994314171&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84994314171&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-6448-2_23

DO - 10.1007/978-1-4939-6448-2_23

M3 - Article

VL - 1493

SP - 311

EP - 319

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -