Antibody-induced modulation of the CD3/T cell receptor complex causes T cell refractoriness by inhibiting the early metabolic steps involved in T cell activation

G. Pantaleo, D. Olive, A. Poggi, T. Pozzan, L. Moretta, A. Moretta

Research output: Contribution to journalArticle

Abstract

We investigated the mechanism involved in T cell unresponsiveness that follows the monoclonal antibody-induced surface modulation of the CD3-TCR complex. We determined whether modulation of CD3-TCR affected the early metabolic steps such a [Ca2+](i) rise and InsP3 formation. A strong inhibition of the increase on [Ca2+](i) mediated by either anti-TCR or anti-CD2 mAbs was detected. In contrast, surface modulation of CD2 molecules did not prevent the [Ca2+](i) increase induced by anti-TCR mAb. Similarly, InsP3 increase was strongly reduced only after modulation of CD3-TCR complex (but not of CD2 molecules). Therefore, it appears that surface modulation of CD3-TCR complex causes T cell refractoriness by inhibiting the very early metabolic events that follow receptor-ligand interactions.

Original languageEnglish
Pages (from-to)619-624
Number of pages6
JournalJournal of Experimental Medicine
Volume166
Issue number2
Publication statusPublished - 1987

ASJC Scopus subject areas

  • Immunology

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