Anticancer and anti-inflammatory properties of ganoderma lucidum extract effects on melanoma and triple-negative breast cancer treatment

A. Barbieri, V. Quagliariello, V. Del Vecchio, M. Falco, A. Luciano, N.J. Amruthraj, G. Nasti, A. Ottaiano, M. Berretta, R.V. Iaffaioli, C. Arra

Research output: Contribution to journalArticlepeer-review


Among the most important traditional medicinal fungi, Ganoderma lucidum has been used as a therapeutic agent for the treatment of numerous diseases, including cancer, in Oriental countries. The aim of this study is to investigate the anti-inflammatory, anticancer and anti-metastatic activities of Ganoderma lucidum extracts in melanoma and triple-negative breast cancer cells. Ganoderma lucidum extracts were prepared by using common organic solvents; MDA-MB 231 and B16-F10 cell lines were adopted as cellular models for triple-negative breast cancer and melanoma and characterized for cell viability, wound-healing assay and measurement of cytokines secreted by cancer cells under pro-inflammatory conditions (incubation with lipopolysaccharide, LPS) and pretreatment with Ganoderma lucidum extract at different concentrations. Our study demonstrates, for the first time, how Ganoderma lucidum extracts can significantly inhibit the release of IL-8, IL-6, MMP-2 and MMP-9 in cancer cells under pro-inflammatory condition. Interestingly, Ganoderma lucidum extracts significantly also decrease the viability of both cancer cells in a time- and concentration-dependent manner, with abilities to reduce cell migration over time, which is correlated with a lower release of matrix metalloproteases. Taken together, these results indicate the possible use of Ganoderma lucidum extract for the therapeutic management of melanoma and human triple-negative breast cancer. © 2017 by the authors. Licensee MDPI, Basel, Switzerland.
Original languageEnglish
Issue number3
Publication statusPublished - 2017


  • Breast cancer
  • Cell viability
  • Curcumin
  • Cytokines
  • Ganoderma lucidum
  • Inflammation
  • Melanoma
  • Ganoderma lucidum extract
  • gelatinase A
  • gelatinase B
  • interleukin 6
  • interleukin 8
  • lipopolysaccharide
  • antiinflammatory agent
  • antineoplastic agent
  • animal cell
  • antiinflammatory activity
  • antineoplastic activity
  • Article
  • cell migration
  • cell proliferation
  • cell survival
  • cell viability
  • concentration response
  • controlled study
  • enzyme linked immunosorbent assay
  • human
  • human cell
  • melanoma
  • MTT assay
  • nonhuman
  • triple negative breast cancer
  • wound healing
  • animal
  • C57BL mouse
  • chemistry
  • drug effects
  • metabolism
  • mouse
  • pathology
  • Triple Negative Breast Neoplasms
  • tumor cell line
  • Animals
  • Anti-Inflammatory Agents
  • Antineoplastic Agents
  • Cell Line, Tumor
  • Cell Proliferation
  • Humans
  • Interleukin-6
  • Interleukin-8
  • Matrix Metalloproteinase 2
  • Matrix Metalloproteinase 9
  • Mice
  • Mice, Inbred C57BL
  • Reishi


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