Anticardiolipin and anti-β2glycoprotein I immunoassays in the diagnosis of antiphospholipid syndrome

A. Tincani, G. Balestrieri, L. Spatola, M. Cinquini, P. L. Meroni, R. A S Roubey

Research output: Contribution to journalArticlepeer-review

Abstract

Autoantibodies directed to phospholipids or to phospholipid binding proteins are now studied using a growing number of laboratory tests. However, the history of the interest in this area goes back to the identification of the so-called false positive reactions in the non-treponemal serological test for syphilis (STS) and to the subsequent description of an in vitro coagulation defect called lupus anticoagulant (LAC). In the 1980s the introduction of the anticardiolipin antibody (aCL) immunoassay was a determining factor in the definition of the antiphospholipid syndrome (AIDS). In addition, lupus prone mice spontaneously producing aCL antibodies and normal mice passively infused with these antibodies provided useful models for the study of the pathogenic role of aPL. When in 1990 a phospholipid binding protein (β2glycoprotein I, β2GPI) was identified as the cofactor required for aCL antibody binding, the true antigenic target of the antibodies was first discussed. Soon afterwards an anti-β2GPI ELISA was developed that has proved to be of great clinical significance. We will discuss here the similarities and differences between these various assays (LAC, aCL, and anti-β2GPI), focusing on their specificity, sensitivity and practical applications.

Original languageEnglish
Pages (from-to)396-402
Number of pages7
JournalClinical and Experimental Rheumatology
Volume16
Issue number4
Publication statusPublished - 1998

Keywords

  • Anti-β glycoprotein I antibodies
  • Anticardiolipin antibodies
  • Antiphospholipid antibodies
  • Antiphospholipid syndrome
  • Beta β glycoprotein I
  • Lupus anticoagulant
  • Systemic lupus erythematosus

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

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