Antidegenerative effects of Mg2+-valproate in cultured cerebellar neurons

V. Bruno; M. A. Sortino; U. Scapagnini; F. Nicoletti; P. L. Canonico

Antidegenerative effects of Mg2+-valproate in cultured cerebellar neurons

V. Bruno, M. A. Sortino, U. Scapagnini, F. Nicoletti, P. L. Canonico

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Abstract

We have investigated in the present study the effect of Mg²⁺-valproate on necrotic degeneration induced by an excitotoxic insult in primary culture of cerebellar neurons, that is an homogeneous population of glutamatergic neurons. Mg²⁺-valproate protected cultures against glutamate-induced neurotoxicity, acting as an indirect N-methyl-D-aspartate (NMDA) receptor antagonist, thus reducing free radical formation and affecting the biochemical parameters (i.e. ⁴⁵Ca²⁺-influx, cyclic GMP formation, inositol phospholipid hydrolysis and protein kinase C translocation) that undergo modifications following NMDA receptor activation in cerebellar granule cells.

Original language	English
Pages (from-to)	121-130
Number of pages	10
Journal	Functional Neurology
Volume	10
Issue number	3
Publication status	Published - 1995

Keywords

Cerebellar granule cells
Excitotoxicity
Glutamate
Mg-valproic acid

ASJC Scopus subject areas

Clinical Neurology
Neuroscience(all)

Cite this

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abstract = "We have investigated in the present study the effect of Mg2+-valproate on necrotic degeneration induced by an excitotoxic insult in primary culture of cerebellar neurons, that is an homogeneous population of glutamatergic neurons. Mg2+-valproate protected cultures against glutamate-induced neurotoxicity, acting as an indirect N-methyl-D-aspartate (NMDA) receptor antagonist, thus reducing free radical formation and affecting the biochemical parameters (i.e. 45Ca2+-influx, cyclic GMP formation, inositol phospholipid hydrolysis and protein kinase C translocation) that undergo modifications following NMDA receptor activation in cerebellar granule cells.",

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AU - Bruno, V.

AU - Sortino, M. A.

AU - Scapagnini, U.

AU - Nicoletti, F.

AU - Canonico, P. L.

PY - 1995

Y1 - 1995

N2 - We have investigated in the present study the effect of Mg2+-valproate on necrotic degeneration induced by an excitotoxic insult in primary culture of cerebellar neurons, that is an homogeneous population of glutamatergic neurons. Mg2+-valproate protected cultures against glutamate-induced neurotoxicity, acting as an indirect N-methyl-D-aspartate (NMDA) receptor antagonist, thus reducing free radical formation and affecting the biochemical parameters (i.e. 45Ca2+-influx, cyclic GMP formation, inositol phospholipid hydrolysis and protein kinase C translocation) that undergo modifications following NMDA receptor activation in cerebellar granule cells.

AB - We have investigated in the present study the effect of Mg2+-valproate on necrotic degeneration induced by an excitotoxic insult in primary culture of cerebellar neurons, that is an homogeneous population of glutamatergic neurons. Mg2+-valproate protected cultures against glutamate-induced neurotoxicity, acting as an indirect N-methyl-D-aspartate (NMDA) receptor antagonist, thus reducing free radical formation and affecting the biochemical parameters (i.e. 45Ca2+-influx, cyclic GMP formation, inositol phospholipid hydrolysis and protein kinase C translocation) that undergo modifications following NMDA receptor activation in cerebellar granule cells.

KW - Cerebellar granule cells

KW - Excitotoxicity

KW - Glutamate

KW - Mg-valproic acid

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Antidegenerative effects of Mg2+-valproate in cultured cerebellar neurons

Abstract

Keywords

ASJC Scopus subject areas

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