Antigen-binding cells following contact-sensitization detected by rosette-formation: Characterization of the receptors by inhibition of rosette-forming cells with anti-rabbit allotype and anti-class specific antisera

M. Ferrarini, A. Munro, S. P. Kent, B. Gurner, R. R A Coombs

Research output: Contribution to journalArticlepeer-review

Abstract

Antigen-binding cells from auricular lymph nodes of rabbits skin sensitized with DNFB were detected by rosette formation. Electron microscopy studies on the morphology of the RFC showed that the rosettes were formed around lymphoblasts and small lymphocytes only. Inhibition experiments of RFCs by anti-allo-type and anti-class specific antisera indicated that the receptors of the RFC were immunoglobulin, predominantly of the IgM class. The lack of antibody production, as shown by the absence of plaque-forming cells in the lymph nodes would suggest that the RFCs are possibly the cells involved in a delayed hypersensitivity response. However, the lack of membrane antigenic markers for T and B cells in rabbits makes the interpretation of these results difficult.

Original languageEnglish
Pages (from-to)321-337
Number of pages17
JournalInternational Archives of Allergy and Immunology
Volume44
Issue number3
DOIs
Publication statusPublished - 1973

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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