Organ cultures of 17-day-old chick embryo spleens were made in the presence of a soluble antigen (bovine serum albumin). After 24 h of incubation with 10 μ/ml of antigen, mast cells (MC) start to differentiate from some unknown precursor cells, and their total number increased progressively till 72 h. No mitoses were observed in MCs during the whole period of incubation. Histochemical and ultrastructural evidence of MC differentiation is presented and the possible inductive mechanisms are briefly discussed.
ASJC Scopus subject areas
- Cell Biology