Antigen preparation for immunological studies in systemic autoimmune diseases

Sandra Zampieri, Anna Ghirardello, Katia Rossini, Luca Iaccarino, Nicola Bassi, Fabiola Atzeni, Piercarlo Sarzi-Puttini, Andrea Doria

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Systemic autoimmune diseases are characterized by the presence in patient sera of high titers of autoantibodies directed against self-antigens. The characterization of specific autoantigen-autoantibody system is very important for research as well as for diagnostic tools and several techniques can be employed in such immunological studies. In this review an overview of the different biochemical methods of antigen preparation and the specific methodological applications is given, also describing potential critical aspects and benefits of the different protocols.

Original languageEnglish
Title of host publicationAnnals of the New York Academy of Sciences
Pages193-202
Number of pages10
Volume1109
DOIs
Publication statusPublished - Aug 2007

Publication series

NameAnnals of the New York Academy of Sciences
Volume1109
ISSN (Print)00778923
ISSN (Electronic)17496632

Keywords

  • Antigenic source
  • Autoantigens
  • Cell extracts
  • Immunoassays
  • Lymphocytes
  • TRIzol

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Zampieri, S., Ghirardello, A., Rossini, K., Iaccarino, L., Bassi, N., Atzeni, F., Sarzi-Puttini, P., & Doria, A. (2007). Antigen preparation for immunological studies in systemic autoimmune diseases. In Annals of the New York Academy of Sciences (Vol. 1109, pp. 193-202). (Annals of the New York Academy of Sciences; Vol. 1109). https://doi.org/10.1196/annals.1398.023