Antigen preparation for immunological studies in systemic autoimmune diseases

Sandra Zampieri, Anna Ghirardello, Katia Rossini, Luca Iaccarino, Nicola Bassi, Fabiola Atzeni, Piercarlo Sarzi-Puttini, Andrea Doria

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Systemic autoimmune diseases are characterized by the presence in patient sera of high titers of autoantibodies directed against self-antigens. The characterization of specific autoantigen-autoantibody system is very important for research as well as for diagnostic tools and several techniques can be employed in such immunological studies. In this review an overview of the different biochemical methods of antigen preparation and the specific methodological applications is given, also describing potential critical aspects and benefits of the different protocols.

Original languageEnglish
Title of host publicationAnnals of the New York Academy of Sciences
Number of pages10
Publication statusPublished - Aug 2007

Publication series

NameAnnals of the New York Academy of Sciences
ISSN (Print)00778923
ISSN (Electronic)17496632


  • Antigenic source
  • Autoantigens
  • Cell extracts
  • Immunoassays
  • Lymphocytes
  • TRIzol

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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