Antigen-Specific Immunity in Neuroblastoma Patients: Antibody and T-Cell Recognition of NY-ESO-1 Tumor Antigen

Monica Rodolfo, Roberto Luksch, Elisabeth Stockert, Yao Tseng Chen, Paola Collini, Tiziana Ranzani, Claudia Lombardo, Piero Dalerba, Licia Rivoltini, Flavio Arienti, Franca Fossati-Bellani, Lloyd J. Old, Giorgio Parmiani, Chiara Castelli

Research output: Contribution to journalArticlepeer-review

Abstract

Neuroblastoma cells have been shown to express molecularly defined tumor-associated antigens, which could represent potential targets of T and/or B cell-mediated immunity. However, the existence of a spontaneous immune response to such tumor antigens in neuroblastoma patients has yet to be investigated. In the present work we addressed the issue of whether NY-ESO-1, a germ cell antigen aberrantly expressed in different tumor types, is expressed by neuroblastoma cells and may represent a target for humoral and/or cellular immune responses in neuroblastoma patients. We found that a large fraction of neuroblastoma biopsies, independently from the clinical stage and degree of tumor cell differentiation, expressed significant levels of NY-ESO-1 as assessed by reverse transcription-PCR and immunohistochemistry. NY-ESO-1-specific IgG antibodies were detected in the sera of 10% of neuroblastoma patients with stage III or IV disease, but not in patients in clinical remission or with earlier stages. This suggests that antibody production occurred as a late event in the course of disease. NY-ESO-1-specific immune responses were observed for CD4+ and CD8+ T cells from peripheral blood lymphocytes in 4 of 8 neuroblastoma patients, as detected by IFN-τ enzyme-linked immunospot assay after in vitro stimulation either with the NY-ESO-1 recombinant protein or with the HLA-A2-restricted peptide NY-ESO1157-167. NY-ESO-1-specific CD4+ and CD8 + T cells were also able to recognize NY-ESO-1 expressing neuroblastoma cells. The presence of T cells specific for NY-ESO-1 antigen was not associated with the stage of disease, or to the presence or absence of NY-ESO-1 specific antibodies. We conclude that NY-ESO-1 is an immunogenic antigen in neuroblastoma patients and represents a candidate target for immune-based therapy.

Original languageEnglish
Pages (from-to)6948-6955
Number of pages8
JournalCancer Research
Volume63
Issue number20
Publication statusPublished - Oct 15 2003

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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