Antigenic and immunogenic mimicry of the HER2/neu oncoprotein by phage-displayed peptides

Rosaria Orlandi, Sylvie Ménard, Maria I. Colnaghi, Cinda M. Boyer, Franco Felici

Research output: Contribution to journalArticle

Abstract

To recover peptides that antigenically and immunogenically mimic the p185(HER2) oncoprotein, we selected the phage-peptide libraries pVIII-9aa and pVllI-9aa. Cys using murine monoclonal antibodies (mAb) MGr2 and MGr6, directed against two distinct epitopes of the p185(HER2) extracellular domain. Phage-displayed peptides containing consensus amino acid motifs were recovered and shown to compete specifically for mAb binding on tumor cells that overexpress p185(HER2). The deduced amino acid sequence of the peptides suggests that both epitopes defined by the mAb on p185(HER2) are discontinuous and that hydrophobic interactions are involved in binding with the mAb. A phage clone displaying the GPLDSLFAQ peptide elicited a specific immune response against the p185(HER2) in BALB/c mice, demonstrating that this phage-displayed peptide represents an immunological equivalent of the MGr2 epitope on p185(HER2) and might be used as a substitute for this oncoprotein in in vitro and in vivo immunological studies.

Original languageEnglish
Pages (from-to)2868-2873
Number of pages6
JournalEuropean Journal of Immunology
Volume24
Issue number11
DOIs
Publication statusPublished - Nov 1994

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Keywords

  • Epitope libraries
  • Filamentous phage
  • HER2
  • Neu
  • Peptide mimics
  • Tumor

ASJC Scopus subject areas

  • Immunology

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