Abstract
Comparison between antigens solubilized from MOPC-460 mouse plasmacytoma cell membranes by means of three methods (treatment with a surfactant: sodium desoxycholate (DOC); high ionic strength, using 3 M potassium chloride (3 M KCl); insolubilized papain (PAP) digestion) is reported. DOC displayed the greatest quantitative efficiency and brought more than 80 per cent of the membrane proteins in solution, as well as about 15 per cent of the protein-bound sugars. 3M KCl and papain solubilized 16 per cent and 10 per cent proteins and 3 per cent and 5 per cent sugars respectively. SDS-acrylamide electrophoresis showed that DOC also solubilized the greatest number of proteins and demonstrated the disadvantages associated with PAP digestion. In the rabbit, however, DOC-solubilized proteins displayed poor immunogenicity. In particular, precipitating antibodies against membrane antigens that were not equally solubilized by 3 M KCl could not be obtained. It is deduced from what is known of surfactant mechanisms that DOC solubilizes both 'integral' and 'extrinsic' membrane proteins, whereas the ionic strength acts primarily on the latter. Cross-adsorption experiments with the antisera produced against antigens solubilized with the two agents showed that the great majority of the antibodies induced against antigens solubilized from cell membranes were directed against 'extrinsic' proteins.
| Original language | English |
|---|---|
| Pages (from-to) | 9-17 |
| Number of pages | 9 |
| Journal | Immunochemistry |
| Volume | 12 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1975 |
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ASJC Scopus subject areas
- Molecular Biology
- Immunology
Cite this
Antigenic and immunogenic properties of membrane proteins solubilized by sodium desoxycholate, papain digestion or high ionic strength. / Prat, M.; Tarone, G.; Comoglio, P. M.
In: Immunochemistry, Vol. 12, No. 1, 1975, p. 9-17.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Antigenic and immunogenic properties of membrane proteins solubilized by sodium desoxycholate, papain digestion or high ionic strength
AU - Prat, M.
AU - Tarone, G.
AU - Comoglio, P. M.
PY - 1975
Y1 - 1975
N2 - Comparison between antigens solubilized from MOPC-460 mouse plasmacytoma cell membranes by means of three methods (treatment with a surfactant: sodium desoxycholate (DOC); high ionic strength, using 3 M potassium chloride (3 M KCl); insolubilized papain (PAP) digestion) is reported. DOC displayed the greatest quantitative efficiency and brought more than 80 per cent of the membrane proteins in solution, as well as about 15 per cent of the protein-bound sugars. 3M KCl and papain solubilized 16 per cent and 10 per cent proteins and 3 per cent and 5 per cent sugars respectively. SDS-acrylamide electrophoresis showed that DOC also solubilized the greatest number of proteins and demonstrated the disadvantages associated with PAP digestion. In the rabbit, however, DOC-solubilized proteins displayed poor immunogenicity. In particular, precipitating antibodies against membrane antigens that were not equally solubilized by 3 M KCl could not be obtained. It is deduced from what is known of surfactant mechanisms that DOC solubilizes both 'integral' and 'extrinsic' membrane proteins, whereas the ionic strength acts primarily on the latter. Cross-adsorption experiments with the antisera produced against antigens solubilized with the two agents showed that the great majority of the antibodies induced against antigens solubilized from cell membranes were directed against 'extrinsic' proteins.
AB - Comparison between antigens solubilized from MOPC-460 mouse plasmacytoma cell membranes by means of three methods (treatment with a surfactant: sodium desoxycholate (DOC); high ionic strength, using 3 M potassium chloride (3 M KCl); insolubilized papain (PAP) digestion) is reported. DOC displayed the greatest quantitative efficiency and brought more than 80 per cent of the membrane proteins in solution, as well as about 15 per cent of the protein-bound sugars. 3M KCl and papain solubilized 16 per cent and 10 per cent proteins and 3 per cent and 5 per cent sugars respectively. SDS-acrylamide electrophoresis showed that DOC also solubilized the greatest number of proteins and demonstrated the disadvantages associated with PAP digestion. In the rabbit, however, DOC-solubilized proteins displayed poor immunogenicity. In particular, precipitating antibodies against membrane antigens that were not equally solubilized by 3 M KCl could not be obtained. It is deduced from what is known of surfactant mechanisms that DOC solubilizes both 'integral' and 'extrinsic' membrane proteins, whereas the ionic strength acts primarily on the latter. Cross-adsorption experiments with the antisera produced against antigens solubilized with the two agents showed that the great majority of the antibodies induced against antigens solubilized from cell membranes were directed against 'extrinsic' proteins.
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UR - http://www.scopus.com/inward/citedby.url?scp=0016426839&partnerID=8YFLogxK
U2 - 10.1016/0019-2791(75)90044-0
DO - 10.1016/0019-2791(75)90044-0
M3 - Article
C2 - 1140821
AN - SCOPUS:0016426839
VL - 12
SP - 9
EP - 17
JO - Immunochemistry
JF - Immunochemistry
SN - 0019-2791
IS - 1
ER -