Antigenic expression changes occurring in adriamycin resistant MCF-7 mammary carcinoma cells

M. Nuti, G. Zupi, I. D'Agnano, V. Turchi, A. Candiloro, L. Frati

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The antigenic phenotypic repertoire of MGF-7 human breast carcinoma cell line variants that display different sensitivity to adriamycin (Adr) was analyzed using monoclonal antibodies (MoAbs) recognizing five different tumor associated antigens (TAAs) and the external domain of the epidermal growth factor receptor (EGF-R). ELISA and cytofluorimetry determinations were used and results indicate a diminished expression of one antigenic determinant of the carcinoembryonic antigen (CEA) molecule, the disappearance of all the other TAAs and the de novo expression of the EGF-R in the MCF-7 AdrR (IC50/Adr:10 uM). Treatment with recombinant alfa-Interferon (alfa-IFN) did not enhance antigenic expression in MCF-7 AdrR cells.

Original languageEnglish
Pages (from-to)1225-1230
Number of pages6
JournalAnticancer Research
Volume11
Issue number3
Publication statusPublished - 1991

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Neoplasm Antigens
Epidermal Growth Factor Receptor
Doxorubicin
Breast Neoplasms
Carcinoembryonic Antigen
MCF-7 Cells
Interferon-alpha
Inhibitory Concentration 50
Epitopes
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Cell Line
Therapeutics

Keywords

  • breast cancer
  • drug resistance
  • TAA expression

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Nuti, M., Zupi, G., D'Agnano, I., Turchi, V., Candiloro, A., & Frati, L. (1991). Antigenic expression changes occurring in adriamycin resistant MCF-7 mammary carcinoma cells. Anticancer Research, 11(3), 1225-1230.

Antigenic expression changes occurring in adriamycin resistant MCF-7 mammary carcinoma cells. / Nuti, M.; Zupi, G.; D'Agnano, I.; Turchi, V.; Candiloro, A.; Frati, L.

In: Anticancer Research, Vol. 11, No. 3, 1991, p. 1225-1230.

Research output: Contribution to journalArticle

Nuti, M, Zupi, G, D'Agnano, I, Turchi, V, Candiloro, A & Frati, L 1991, 'Antigenic expression changes occurring in adriamycin resistant MCF-7 mammary carcinoma cells', Anticancer Research, vol. 11, no. 3, pp. 1225-1230.
Nuti, M. ; Zupi, G. ; D'Agnano, I. ; Turchi, V. ; Candiloro, A. ; Frati, L. / Antigenic expression changes occurring in adriamycin resistant MCF-7 mammary carcinoma cells. In: Anticancer Research. 1991 ; Vol. 11, No. 3. pp. 1225-1230.
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