Antigenic profile and functional characterization of human peritoneal macrophages

M. Mottolese, P. G. Natali, G. Atlante, A. Cavallari, F. DiFilippo, S. Ferrone

Research output: Contribution to journalArticlepeer-review

Abstract

The antigenic profile and the functional properties of human peritoneal macrophages have been analyzed by using a panel of monoclonal antibodies (MoAb) and functional assays. All peritoneal macrophages were stained by the anti-class I HLA MoAb Q6/64. Between 40 and 100% of the cells were stained by the anti-HLA-DR + DP MoAb Q2/80, Q5/6, and Q5/13; approximately 80% of the cells were stained by the anti-HLA-DQ MoAb BT3.4, and about 95% were stained by the anti-macrophage MoAb OKM1. Peritoneal macrophages were not stained by the anti-dendritic cells MoAb Ki-M4 or by MoAb to T cell subsets, although all of the MoAb were reactive with the appropriate substrates. More than 60% of the cells expressed Fc receptors and C3 receptors, and displayed phagocytic activity. Peritoneal macrophages were effective in stimulating autologous and allogeneic lymphocytes and in presenting soluble antigens to T cells. These reactions were blocked by the anti-HLA-DR + DP MoAb Q5/13, but were not affected by the anti-dendritic cells MoAb Ki-M4 or by the anti-class I HLA MoAb Q6/64. These results suggest that human peritoneal macrophage preparations, without detectable contamination with dendritic cells, can induce proliferation of autologous and allogeneic T cells, and that class II HLA antigens play a significant role in these phenomena.

Original languageEnglish
Pages (from-to)200-206
Number of pages7
JournalJournal of Immunology
Volume135
Issue number1
Publication statusPublished - 1985

ASJC Scopus subject areas

  • Immunology

Fingerprint

Dive into the research topics of 'Antigenic profile and functional characterization of human peritoneal macrophages'. Together they form a unique fingerprint.

Cite this