Antigenicity and immunogenicity of phage library-selected peptide mimics of the major surface proteophosphoglycan antigens of Entamoeba histolytica

Helen Melzer, Paola Fortugno, Erfan Mansouri, Franco Felici, Alexandra Marinets, Gerhard Wiedermann, Herwig Kollaritsch, Bernd Ulrich Von Specht, Michael Duchêne

Research output: Contribution to journalArticlepeer-review

Abstract

Entamoeba histolytica is the protozoan parasite responsible for intestinal amoebiasis and amoebic liver abscess, which cause significant morbidity and mortality in many countries of the world. Proteophosphoglycans (PPGs, also known as lipophosphoglycans, LPGs, or lipopeptidophosphoglycans, LPPGs) represent dominant surface components of E. histolytica. Passive immunization with a monoclonal antibody (EH5) directed against these components protected SCID mice from amoebic liver abscess, so PPGs might be regarded as vaccine candidates; however, their structure is very complex and only known in part. They are glycosylphosphatidylinositol-linked polypeptides of unknown sequence carrying glycan side-chains linked to serine residues via phosphodiester bonds. In order to identify peptide mimics of the E. histolytica PPG antigens, we screened six different phage-displayed random peptide libraries with the antibody EH5. Various peptide mimics of different length were identified and, in all the peptides, a distinct consensus sequence Gly-Thr-His-Pro-X-Leu could be identified. The phages strongly bound to the antibody, and the natural antigen inhibited binding of the phages to antibody EH5. In addition, several of the phages induced a significant immunoglobulin G response against amoebic antigens in immunized mice.

Original languageEnglish
Pages (from-to)321-328
Number of pages8
JournalParasite Immunology
Volume24
Issue number6
DOIs
Publication statusPublished - 2002

Keywords

  • Entamoeba histolytica
  • Lipophosphoglycan
  • Mimotope
  • Phage display
  • Proteophosphoglycan

ASJC Scopus subject areas

  • Parasitology
  • Immunology

Fingerprint Dive into the research topics of 'Antigenicity and immunogenicity of phage library-selected peptide mimics of the major surface proteophosphoglycan antigens of Entamoeba histolytica'. Together they form a unique fingerprint.

Cite this