Antiinflammatory activity of glucomoringin isothiocyanate in a mouse model of experimental autoimmune encephalomyelitis

Maria Galuppo, Sabrina Giacoppo, Gina Rosalinda De Nicola, Renato Iori, Michele Navarra, Giovanni Enrico Lombardo, Placido Bramanti, Emanuela Mazzon

Research output: Contribution to journalArticle

Abstract

Glucomoringin (4(α-L-rhamnosyloxy)-benzyl glucosinolate) (GMG) is an uncommon member of glucosinolate group belonging to the Moringaceae family, of which Moringa oleifera Lam. is the most widely distributed. Bioactivation of GMG with the enzyme myrosinase forms the corresponding isothiocyanate (4(α-L-rhamnosyloxy)-benzyl isothiocyanate) (GMG-ITC), which can play a key role in antitumoral activity and counteract the inflammatory response. The aim of this study was to assess the effect of GMG-ITC treatment in an experimental mouse model of multiple sclerosis (MS), an inflammatory demyelinating disease with neurodegeneration characterized by demyelinating plaques, neuronal, and axonal loss. For this reason, C57Bl/6 male mice were injected with myelin oligodendrocyte glycoprotein35-55 which is able to evoke an autoimmune response against myelin fibers miming human multiple sclerosis physiopatogenesis. Results clearly showed that the treatment was able to counteract the inflammatory cascade that underlies the processes leading to severe MS. In particular, GMG-ITC was effective against proinflammatory cytokine TNF-α. Oxidative species generation including the influence of iNOS, nitrotyrosine tissue expression and cell apoptotic death pathway was also evaluated resulting in a lower Bax/Bcl-2 unbalance. Taken together, this work adds new interesting properties and applicability of GMG-ITC and this compound can be suggested as a useful drug for the treatment or prevention of MS, at least in association with current conventional therapy.

Original languageEnglish
Pages (from-to)160-174
Number of pages15
JournalFitoterapia
Volume95
DOIs
Publication statusPublished - 2014

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Autoimmune Experimental Encephalomyelitis
Multiple Sclerosis
Anti-Inflammatory Agents
Glucosinolates
Myelin Sheath
Moringa oleifera
Oligodendroglia
Demyelinating Diseases
Autoimmunity
Cell Death
Theoretical Models
Cytokines
4-((alpha-L-rhamnosyloxy)benzyl)isothiocyanate
Enzymes
Pharmaceutical Preparations
glucose-mannose-glucose
Therapeutics

Keywords

  • Cytokines
  • Glucomoringin
  • Inflammation
  • Isothiocyanate
  • Multiple sclerosis

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery
  • Medicine(all)

Cite this

Antiinflammatory activity of glucomoringin isothiocyanate in a mouse model of experimental autoimmune encephalomyelitis. / Galuppo, Maria; Giacoppo, Sabrina; De Nicola, Gina Rosalinda; Iori, Renato; Navarra, Michele; Lombardo, Giovanni Enrico; Bramanti, Placido; Mazzon, Emanuela.

In: Fitoterapia, Vol. 95, 2014, p. 160-174.

Research output: Contribution to journalArticle

Galuppo, Maria ; Giacoppo, Sabrina ; De Nicola, Gina Rosalinda ; Iori, Renato ; Navarra, Michele ; Lombardo, Giovanni Enrico ; Bramanti, Placido ; Mazzon, Emanuela. / Antiinflammatory activity of glucomoringin isothiocyanate in a mouse model of experimental autoimmune encephalomyelitis. In: Fitoterapia. 2014 ; Vol. 95. pp. 160-174.
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