Antimicrobial activity of human pancreatic juice and its interaction with antibiotics

E. Bertazzoni Minelli, A. Benini, C. Bassi, H. Abbas, M. Falconi, F. Locatelli, R. De Marco, P. Pederzoli

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Pancreatic juice (PJ) should be a factor of variability in the antimicrobial activity of antibiotics eliminated by the pancreas during pancreatic infections. We studied its effects on the activity of antimicrobial drugs with different mechanisms of action. Samples of pure PJ were collected from 16 patients with stabilized external pancreatic fistulas. The antimicrobial activity of the juice at different concentrations (from 1.25 to 100%) alone and in combination with mezlocillin, imipenem, ceftriaxone, gentamicin, ofloxacin, and ciprofioxacin was studied by a microbiological method (continuous turbidimetric recording of bacterial growth). The human PJ showed dose-dependent antimicrobial activity that increased directly with the concentration. The activity of the antibiotics at bactericidal concentrations were not modified by the PJ, while the combination with subinhibitory concentrations produced the following variable and different effects: (i) additivity with mezlocillin, ceftriaxone, gentamicin, and ciprofloxacin and autonomy (no interaction) with imipenem and ofloxacin against Providencia rettgeri and (ii) additivity with ceftriaxone, ofloxacin, gentamicin, imipenem, and mezlocillin and autonomy with ciprofloxacin against Escherichia coli. In the presence of PJ, fluoroquinolones showed constant positive effects, while beta-lactams showed more variable antimicrobial activity. Antibiotic concentrations and PJ pharmacodynamics are the main factors determining the final effect of the interaction in vitro. These results may be useful in choosing antibiotics for the treatment of pancreatic infections when they are supplemented with the pharmacokinetic data for each drug.

Original languageEnglish
Pages (from-to)2099-2105
Number of pages7
JournalAntimicrobial Agents and Chemotherapy
Volume40
Issue number9
Publication statusPublished - Sep 1996

Fingerprint

Pancreatic Juice
Human Activities
Mezlocillin
Anti-Bacterial Agents
Ofloxacin
Ceftriaxone
Imipenem
Gentamicins
Ciprofloxacin
Providencia
Cutaneous Fistula
Pancreatic Fistula
Fluoroquinolones
beta-Lactams
Infection
Pharmaceutical Preparations
Pancreas
Pharmacokinetics
Escherichia coli
Growth

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Bertazzoni Minelli, E., Benini, A., Bassi, C., Abbas, H., Falconi, M., Locatelli, F., ... Pederzoli, P. (1996). Antimicrobial activity of human pancreatic juice and its interaction with antibiotics. Antimicrobial Agents and Chemotherapy, 40(9), 2099-2105.

Antimicrobial activity of human pancreatic juice and its interaction with antibiotics. / Bertazzoni Minelli, E.; Benini, A.; Bassi, C.; Abbas, H.; Falconi, M.; Locatelli, F.; De Marco, R.; Pederzoli, P.

In: Antimicrobial Agents and Chemotherapy, Vol. 40, No. 9, 09.1996, p. 2099-2105.

Research output: Contribution to journalArticle

Bertazzoni Minelli, E, Benini, A, Bassi, C, Abbas, H, Falconi, M, Locatelli, F, De Marco, R & Pederzoli, P 1996, 'Antimicrobial activity of human pancreatic juice and its interaction with antibiotics', Antimicrobial Agents and Chemotherapy, vol. 40, no. 9, pp. 2099-2105.
Bertazzoni Minelli E, Benini A, Bassi C, Abbas H, Falconi M, Locatelli F et al. Antimicrobial activity of human pancreatic juice and its interaction with antibiotics. Antimicrobial Agents and Chemotherapy. 1996 Sep;40(9):2099-2105.
Bertazzoni Minelli, E. ; Benini, A. ; Bassi, C. ; Abbas, H. ; Falconi, M. ; Locatelli, F. ; De Marco, R. ; Pederzoli, P. / Antimicrobial activity of human pancreatic juice and its interaction with antibiotics. In: Antimicrobial Agents and Chemotherapy. 1996 ; Vol. 40, No. 9. pp. 2099-2105.
@article{394755430f3142438f271ed0f3c8bbcc,
title = "Antimicrobial activity of human pancreatic juice and its interaction with antibiotics",
abstract = "Pancreatic juice (PJ) should be a factor of variability in the antimicrobial activity of antibiotics eliminated by the pancreas during pancreatic infections. We studied its effects on the activity of antimicrobial drugs with different mechanisms of action. Samples of pure PJ were collected from 16 patients with stabilized external pancreatic fistulas. The antimicrobial activity of the juice at different concentrations (from 1.25 to 100{\%}) alone and in combination with mezlocillin, imipenem, ceftriaxone, gentamicin, ofloxacin, and ciprofioxacin was studied by a microbiological method (continuous turbidimetric recording of bacterial growth). The human PJ showed dose-dependent antimicrobial activity that increased directly with the concentration. The activity of the antibiotics at bactericidal concentrations were not modified by the PJ, while the combination with subinhibitory concentrations produced the following variable and different effects: (i) additivity with mezlocillin, ceftriaxone, gentamicin, and ciprofloxacin and autonomy (no interaction) with imipenem and ofloxacin against Providencia rettgeri and (ii) additivity with ceftriaxone, ofloxacin, gentamicin, imipenem, and mezlocillin and autonomy with ciprofloxacin against Escherichia coli. In the presence of PJ, fluoroquinolones showed constant positive effects, while beta-lactams showed more variable antimicrobial activity. Antibiotic concentrations and PJ pharmacodynamics are the main factors determining the final effect of the interaction in vitro. These results may be useful in choosing antibiotics for the treatment of pancreatic infections when they are supplemented with the pharmacokinetic data for each drug.",
author = "{Bertazzoni Minelli}, E. and A. Benini and C. Bassi and H. Abbas and M. Falconi and F. Locatelli and {De Marco}, R. and P. Pederzoli",
year = "1996",
month = "9",
language = "English",
volume = "40",
pages = "2099--2105",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "9",

}

TY - JOUR

T1 - Antimicrobial activity of human pancreatic juice and its interaction with antibiotics

AU - Bertazzoni Minelli, E.

AU - Benini, A.

AU - Bassi, C.

AU - Abbas, H.

AU - Falconi, M.

AU - Locatelli, F.

AU - De Marco, R.

AU - Pederzoli, P.

PY - 1996/9

Y1 - 1996/9

N2 - Pancreatic juice (PJ) should be a factor of variability in the antimicrobial activity of antibiotics eliminated by the pancreas during pancreatic infections. We studied its effects on the activity of antimicrobial drugs with different mechanisms of action. Samples of pure PJ were collected from 16 patients with stabilized external pancreatic fistulas. The antimicrobial activity of the juice at different concentrations (from 1.25 to 100%) alone and in combination with mezlocillin, imipenem, ceftriaxone, gentamicin, ofloxacin, and ciprofioxacin was studied by a microbiological method (continuous turbidimetric recording of bacterial growth). The human PJ showed dose-dependent antimicrobial activity that increased directly with the concentration. The activity of the antibiotics at bactericidal concentrations were not modified by the PJ, while the combination with subinhibitory concentrations produced the following variable and different effects: (i) additivity with mezlocillin, ceftriaxone, gentamicin, and ciprofloxacin and autonomy (no interaction) with imipenem and ofloxacin against Providencia rettgeri and (ii) additivity with ceftriaxone, ofloxacin, gentamicin, imipenem, and mezlocillin and autonomy with ciprofloxacin against Escherichia coli. In the presence of PJ, fluoroquinolones showed constant positive effects, while beta-lactams showed more variable antimicrobial activity. Antibiotic concentrations and PJ pharmacodynamics are the main factors determining the final effect of the interaction in vitro. These results may be useful in choosing antibiotics for the treatment of pancreatic infections when they are supplemented with the pharmacokinetic data for each drug.

AB - Pancreatic juice (PJ) should be a factor of variability in the antimicrobial activity of antibiotics eliminated by the pancreas during pancreatic infections. We studied its effects on the activity of antimicrobial drugs with different mechanisms of action. Samples of pure PJ were collected from 16 patients with stabilized external pancreatic fistulas. The antimicrobial activity of the juice at different concentrations (from 1.25 to 100%) alone and in combination with mezlocillin, imipenem, ceftriaxone, gentamicin, ofloxacin, and ciprofioxacin was studied by a microbiological method (continuous turbidimetric recording of bacterial growth). The human PJ showed dose-dependent antimicrobial activity that increased directly with the concentration. The activity of the antibiotics at bactericidal concentrations were not modified by the PJ, while the combination with subinhibitory concentrations produced the following variable and different effects: (i) additivity with mezlocillin, ceftriaxone, gentamicin, and ciprofloxacin and autonomy (no interaction) with imipenem and ofloxacin against Providencia rettgeri and (ii) additivity with ceftriaxone, ofloxacin, gentamicin, imipenem, and mezlocillin and autonomy with ciprofloxacin against Escherichia coli. In the presence of PJ, fluoroquinolones showed constant positive effects, while beta-lactams showed more variable antimicrobial activity. Antibiotic concentrations and PJ pharmacodynamics are the main factors determining the final effect of the interaction in vitro. These results may be useful in choosing antibiotics for the treatment of pancreatic infections when they are supplemented with the pharmacokinetic data for each drug.

UR - http://www.scopus.com/inward/record.url?scp=9544227479&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=9544227479&partnerID=8YFLogxK

M3 - Article

C2 - 8878588

AN - SCOPUS:9544227479

VL - 40

SP - 2099

EP - 2105

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 9

ER -