Antimicrobial properties and cytocompatibility of PLGA/Ag nanocomposites

M. Scavone, I. Armentano, E. Fortunati, Francesco Cristofaro, Samantha Mattioli, L. Torre, J. M. Kenny, Marcello Imbriani, Carla Renata Arciola, Livia Visai

Research output: Contribution to journalArticle

Abstract

The purpose of this study was to investigate the antimicrobial properties of multifunctional nanocomposites based on poly(DL-Lactide-co-Glycolide) (PLGA) and increasing concentration of silver (Ag) nanoparticles and their effects on cell viability for biomedical applications. PLGA nanocomposite films, produced by solvent casting with 1 wt%, 3 wt% and 7 wt% of Ag nanoparticles were investigated and surface properties were characterized by atomic force microscopy and contact angle measurements. Antibacterial tests were performed using an Escherichia coli RB and Staphylococcus aureus 8325-4 strains. The cell viability and morphology were performed with a murine fibroblast cell line (L929) and a human osteosarcoma cell line (SAOS-2) by cell viability assay and electron microscopy observations. Matrix protein secretion and deposition were also quantified by enzyme-linked immunosorbent assay (ELISA). The results suggest that the PLGA film morphology can be modified introducing a small percentage of silver nanoparticles, which induce the onset of porous round-like microstructures and also affect the wettability. The PLGA/Ag films having silver nanoparticles of more than 3 wt% showed antibacterial effects against E. coli and S. aureus. Furthermore, silver-containing PLGA films displayed also a good cytocompatibility when assayed with L929 and SAOS-2 cells; indicating the PLGA/3Ag nanocomposite film as a promising candidate for tissue engineering applications.

Original languageEnglish
Article number37
JournalMaterials
Volume9
Issue number1
DOIs
Publication statusPublished - 2016

Fingerprint

Nanocomposites
Cells
Silver
Nanoparticles
Nanocomposite films
Escherichia coli
Assays
Fibroblasts
Angle measurement
Immunosorbents
Tissue engineering
Electron microscopy
Contact angle
Surface properties
Wetting
Atomic force microscopy
Casting
Enzymes
polylactic acid-polyglycolic acid copolymer
Proteins

Keywords

  • Ag nanocomposites
  • Antibacterial activity
  • Cell cytocompatibility

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Scavone, M., Armentano, I., Fortunati, E., Cristofaro, F., Mattioli, S., Torre, L., ... Visai, L. (2016). Antimicrobial properties and cytocompatibility of PLGA/Ag nanocomposites. Materials, 9(1), [37]. https://doi.org/10.3390/ma9010037

Antimicrobial properties and cytocompatibility of PLGA/Ag nanocomposites. / Scavone, M.; Armentano, I.; Fortunati, E.; Cristofaro, Francesco; Mattioli, Samantha; Torre, L.; Kenny, J. M.; Imbriani, Marcello; Arciola, Carla Renata; Visai, Livia.

In: Materials, Vol. 9, No. 1, 37, 2016.

Research output: Contribution to journalArticle

Scavone, M, Armentano, I, Fortunati, E, Cristofaro, F, Mattioli, S, Torre, L, Kenny, JM, Imbriani, M, Arciola, CR & Visai, L 2016, 'Antimicrobial properties and cytocompatibility of PLGA/Ag nanocomposites', Materials, vol. 9, no. 1, 37. https://doi.org/10.3390/ma9010037
Scavone M, Armentano I, Fortunati E, Cristofaro F, Mattioli S, Torre L et al. Antimicrobial properties and cytocompatibility of PLGA/Ag nanocomposites. Materials. 2016;9(1). 37. https://doi.org/10.3390/ma9010037
Scavone, M. ; Armentano, I. ; Fortunati, E. ; Cristofaro, Francesco ; Mattioli, Samantha ; Torre, L. ; Kenny, J. M. ; Imbriani, Marcello ; Arciola, Carla Renata ; Visai, Livia. / Antimicrobial properties and cytocompatibility of PLGA/Ag nanocomposites. In: Materials. 2016 ; Vol. 9, No. 1.
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