Antimicrobial properties of distinctin in an experimental model of MRSA-infected wounds

O. Simonetti, O. Cirioni, R. Ghiselli, G. Goteri, F. Orlando, L. Monfregola, S. De Luca, A. Zizzi, C. Silvestri, G. Veglia, A. Giacometti, M. Guerrieri, A. Offidani, A. Scaloni

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of this study was to evaluate the efficacy of distinctin in the management of cutaneous methicillin-resistant Staphylococcus aureus (MRSA) wound infections in an experimental mouse model. Wounds, made in the panniculus carnosus of BALB/c mice, were inoculated with 5×107 colony-forming units (CFU) of MRSA. Mice were treated with topical distinctin (1 mg/kg of body weight), topical teicoplanin (7 mg/kg of body weight), intraperitoneal teicoplanin (7 mg/kg of body weight); topical teicoplanin and daily intraperitoneal teicoplanin; topical distinctin and daily intraperitoneal teicoplanin. Bacterial cultures of excised tissues and histological examination of microvessel density and of vascular endothelial growth factor (VEGF) expression were studied. It was found that topical distinctin combined with parenteral teicoplanin inhibited bacterial growth to levels comparable with those observed in uninfected animals. Wounded areas of animals treated with distinctin were characterized by a more mature granulation tissue, with a more organized and denser type of connective tissue, compared to mice treated only with teicoplanin. Treatment with topical distinctin had a significant impact on VEGF expression and microvessel density. The combined use of distinctin with teicoplanin may be useful in the management of infected wounds by significantly inhibiting bacterial growth and accelerating the repair process.

Original languageEnglish
Pages (from-to)3047-3055
Number of pages9
JournalEuropean Journal of Clinical Microbiology and Infectious Diseases
Volume31
Issue number11
DOIs
Publication statusPublished - Nov 2012

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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