Antimicrobial susceptibility of clinical isolates of Enterobacteriaceae producing complex β-lactamase patterns including extended-spectrum enzymes

Bernardetta Segatore; Domenico Setacci; Mariagrazia Perilli; Luigi Franchino; Nicola Franceschini; Alessio Agnifili; Gian Maria Rossolini; Gianfranco Amicosante

doi:10.1016/j.ijantimicag.2003.10.011

Antimicrobial susceptibility of clinical isolates of Enterobacteriaceae producing complex β-lactamase patterns including extended-spectrum enzymes

Bernardetta Segatore, Domenico Setacci, Mariagrazia Perilli, Luigi Franchino, Nicola Franceschini, Alessio Agnifili, Gian Maria Rossolini, Gianfranco Amicosante

Fondazione Don Carlo Gnocchi Onlus

Research output: Contribution to journal › Article › peer-review

Abstract

The antimicrobial susceptibility of 103 clinical isolates of Enterobacteriaceae to 11 antibiotics, was investigated, using a conventional inoculum size (5×10⁵ CFU) and a higher inoculum size (5×10⁸ CFU). All the isolates produced complex β-lactamase patterns, including an extended-spectrum β-lactamase (ESBL) of the TEM- or SHV-type plus other enzymes (a TEM-type or an SHV-type non-ESBL and/or a class C enzyme). The following repertoire of ESBLs was produced by the isolates: TEM-15, TEM-19, TEM-26, TEM-52, TEM-72, TEM-87, TEM-92, SHV-2a, SHV-5 and SHV-12, as assessed by sequencing. Production of the other enzymes was showed by analytical isoelectric focusing. Overall, meropenem was the most active agent and less influenced by inoculum size, while other β-lactams showed a lower activity and a significant inoculum size effect. In conclusion, from its in vitro performance, meropenem could be considered as the last resource drug against strains producing complex β-lactamase patterns including an ESBL.

Original language	English
Pages (from-to)	480-486
Number of pages	7
Journal	International Journal of Antimicrobial Agents
Volume	23
Issue number	5
DOIs	https://doi.org/10.1016/j.ijantimicag.2003.10.011
Publication status	Published - May 2004

Keywords

β-Lactam antibiotics
ESBL
Resistance

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Microbiology
Parasitology
Virology
Immunology and Allergy
Infectious Diseases

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Segatore, B., Setacci, D., Perilli, M., Franchino, L., Franceschini, N., Agnifili, A., Rossolini, G. M., & Amicosante, G. (2004). Antimicrobial susceptibility of clinical isolates of Enterobacteriaceae producing complex β-lactamase patterns including extended-spectrum enzymes. International Journal of Antimicrobial Agents, 23(5), 480-486. https://doi.org/10.1016/j.ijantimicag.2003.10.011

Antimicrobial susceptibility of clinical isolates of Enterobacteriaceae producing complex β-lactamase patterns including extended-spectrum enzymes. / Segatore, Bernardetta; Setacci, Domenico; Perilli, Mariagrazia; Franchino, Luigi; Franceschini, Nicola; Agnifili, Alessio; Rossolini, Gian Maria; Amicosante, Gianfranco.

In: International Journal of Antimicrobial Agents, Vol. 23, No. 5, 05.2004, p. 480-486.

Research output: Contribution to journal › Article › peer-review

Segatore, B, Setacci, D, Perilli, M, Franchino, L, Franceschini, N, Agnifili, A, Rossolini, GM & Amicosante, G 2004, 'Antimicrobial susceptibility of clinical isolates of Enterobacteriaceae producing complex β-lactamase patterns including extended-spectrum enzymes', International Journal of Antimicrobial Agents, vol. 23, no. 5, pp. 480-486. https://doi.org/10.1016/j.ijantimicag.2003.10.011

Segatore, Bernardetta ; Setacci, Domenico ; Perilli, Mariagrazia ; Franchino, Luigi ; Franceschini, Nicola ; Agnifili, Alessio ; Rossolini, Gian Maria ; Amicosante, Gianfranco. / Antimicrobial susceptibility of clinical isolates of Enterobacteriaceae producing complex β-lactamase patterns including extended-spectrum enzymes. In: International Journal of Antimicrobial Agents. 2004 ; Vol. 23, No. 5. pp. 480-486.

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abstract = "The antimicrobial susceptibility of 103 clinical isolates of Enterobacteriaceae to 11 antibiotics, was investigated, using a conventional inoculum size (5×105 CFU) and a higher inoculum size (5×108 CFU). All the isolates produced complex β-lactamase patterns, including an extended-spectrum β-lactamase (ESBL) of the TEM- or SHV-type plus other enzymes (a TEM-type or an SHV-type non-ESBL and/or a class C enzyme). The following repertoire of ESBLs was produced by the isolates: TEM-15, TEM-19, TEM-26, TEM-52, TEM-72, TEM-87, TEM-92, SHV-2a, SHV-5 and SHV-12, as assessed by sequencing. Production of the other enzymes was showed by analytical isoelectric focusing. Overall, meropenem was the most active agent and less influenced by inoculum size, while other β-lactams showed a lower activity and a significant inoculum size effect. In conclusion, from its in vitro performance, meropenem could be considered as the last resource drug against strains producing complex β-lactamase patterns including an ESBL.",

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