Antineoplastic activity of 2-methoxyestradiol in human pancreatic and gastric cancer cells with different multidrug-resistant phenotypes

Guido Schumacher, Janine Hoffmann, Thorsten Cramer, Antonino Spinelli, Dietmar Jacob, Marcus Bahra, Johann Pratschke, Robert Pfitzmann, Sven Schmidt, Hermann Lage

Research output: Contribution to journalArticlepeer-review

Abstract

Background and Aim: Chemoresistance often leads to loss of the last treatment option for cancer. 2-Methoxyestradiol (2-ME2) has been shown to inhibit tumor growth. The aim was to examine the efficacy of 2-ME2 on multidrug-resistant human cells from pancreatic and gastric cancer. Methods: We investigated the impact of 2-ME2 on multidrug-resistant cells derived from human pancreatic and gastric cancer cells that were positive or negative for the MDR1-gene. Results: In pancreatic cancer cells, growth inhibition was 57% in parental, 72% in MDR1-negative and 87% in MDR1-positive cells after 1 μmol/L 2-ME2. In gastric cancer cells we found a growth inhibition of 75% in parental, 82% in MDR1-positive and 95% in MDR1-negative cells. Strong induction of apoptosis was induced after a low dose of 2-ME2. No significant difference in the amount of apoptotic cells was observed between parental and multidrug-resistant cells of both tumor types. The number of apoptotic cells after 2-ME2 ranged from 7.5% in parental gastric cancer cells to 20.1% in MDR1-negative gastric cancer cells. Conclusion: 2-ME2 may therefore have clinical application for chemoresistant cancer.

Original languageEnglish
Pages (from-to)1469-1473
Number of pages5
JournalJournal of Gastroenterology and Hepatology
Volume22
Issue number9
DOIs
Publication statusPublished - 2007

Keywords

  • 2-methoxyestradiol
  • Gastric cancer
  • Multidrug resistance
  • Pancreatic cancer

ASJC Scopus subject areas

  • Gastroenterology
  • Hepatology

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