Antineoplastic activity of povidone-iodine on different mesothelioma cell lines: Results of in vitro study

Alfonso Fiorelli, Francesca Pentimalli, Vittorio D'urso, Domenico Di Marzo, Iris Maria Forte, Antonio Giordano, Marina Di Domenico, Marina Accardo, Umberto Di Serio, Mario Santini

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective: Povidone-iodine (PVP-I) or Betadine, owing to its antineoplastic activity, is also used as an adjuvant during intra-abdominal or intrathoracic surgery. However, the protocol of PVP-I administration has not been optimized to achieve the best antitumoural efficacy. We aimed to determine the optimal concentration of PVP-I, the time of incubation and the mechanism of cell death by analysing the effect of different doses and time of administration of PVP-I on the cell viability of different mesothelioma cell lines. Methods: Four different cell lines (MET 5A/normal mesothelium; H2052/sarcomatoid mesothelioma; ISTMES2/epithelial mesothelioma; MSTO/biphasic mesothelioma) were incubated with increasing concentrations of diluted PVP-I (0.0001; 0.001; 0.01; 0.1; 1%) for 5, 10, 30, 60 min and 24 h, respectively. Cell viability was determined using cell direct cytotoxicity assay and cell death was determined through flow cytometry assay analysis. The superoxide dismutase activity was assessed functionally through a specific inhibitor to evaluate the mechanism of cell death. Results: The antiproliferative effect of PVP-I varied largely among different cell lines in a dose- and time-dependent manner. At 0.1% concentration for 10 min of incubation, the percentage of viable cells was 0.5 ± 0.1; 0.8 ± 0.5 and 0% (P <0.01) for MET5A, ISTMES2 and MSTO, respectively. Conversely, the same concentration did not significantly affect the H2052 cell line which was completely suppressed at a 1% concentration of PVP-I. Double staining of Annexin V and DNA showed that PVP-I induced cell death in all four cell lines via necrosis depending on PVP-I concentration. However, H2052 was found to be more resistant than MSTO, ISTMES2 and MET 5A cells lines. The activity of superoxide dismutase was significantly inhibited in all cell lines. Conclusions: Our results confirmed the anti-neoplastic activity of PVP-I especially on ISTMES2 and MSTO cell lines. With respect to chemotherapy pleural irrigation, washing with PVP-I is cost-effective and easy. If confirmed by larger studies, our findings suggest that the intrapleural irrigation with PVP-I (0.1% concentration for 10 min) in patients with epithelial or biphasic mesothelioma undergoing cytoreductive surgery might be applied in thoracic surgery practice to prevent neoplastic cell growth.

Original languageEnglish
Article numberezt534
Pages (from-to)993-1000
Number of pages8
JournalEuropean Journal of Cardio-thoracic Surgery
Volume45
Issue number6
DOIs
Publication statusPublished - 2014

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Povidone-Iodine
Mesothelioma
Antineoplastic Agents
Cell Line
Cell Death
In Vitro Techniques
Superoxide Dismutase
Cell Survival
Annexin A5

Keywords

  • Betadine
  • Mesothelioma
  • Povidone-iodide

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery
  • Pulmonary and Respiratory Medicine
  • Medicine(all)

Cite this

Fiorelli, A., Pentimalli, F., D'urso, V., Di Marzo, D., Forte, I. M., Giordano, A., ... Santini, M. (2014). Antineoplastic activity of povidone-iodine on different mesothelioma cell lines: Results of in vitro study. European Journal of Cardio-thoracic Surgery, 45(6), 993-1000. [ezt534]. https://doi.org/10.1093/ejcts/ezt534

Antineoplastic activity of povidone-iodine on different mesothelioma cell lines : Results of in vitro study. / Fiorelli, Alfonso; Pentimalli, Francesca; D'urso, Vittorio; Di Marzo, Domenico; Forte, Iris Maria; Giordano, Antonio; Di Domenico, Marina; Accardo, Marina; Di Serio, Umberto; Santini, Mario.

In: European Journal of Cardio-thoracic Surgery, Vol. 45, No. 6, ezt534, 2014, p. 993-1000.

Research output: Contribution to journalArticle

Fiorelli, A, Pentimalli, F, D'urso, V, Di Marzo, D, Forte, IM, Giordano, A, Di Domenico, M, Accardo, M, Di Serio, U & Santini, M 2014, 'Antineoplastic activity of povidone-iodine on different mesothelioma cell lines: Results of in vitro study', European Journal of Cardio-thoracic Surgery, vol. 45, no. 6, ezt534, pp. 993-1000. https://doi.org/10.1093/ejcts/ezt534
Fiorelli, Alfonso ; Pentimalli, Francesca ; D'urso, Vittorio ; Di Marzo, Domenico ; Forte, Iris Maria ; Giordano, Antonio ; Di Domenico, Marina ; Accardo, Marina ; Di Serio, Umberto ; Santini, Mario. / Antineoplastic activity of povidone-iodine on different mesothelioma cell lines : Results of in vitro study. In: European Journal of Cardio-thoracic Surgery. 2014 ; Vol. 45, No. 6. pp. 993-1000.
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abstract = "Objective: Povidone-iodine (PVP-I) or Betadine, owing to its antineoplastic activity, is also used as an adjuvant during intra-abdominal or intrathoracic surgery. However, the protocol of PVP-I administration has not been optimized to achieve the best antitumoural efficacy. We aimed to determine the optimal concentration of PVP-I, the time of incubation and the mechanism of cell death by analysing the effect of different doses and time of administration of PVP-I on the cell viability of different mesothelioma cell lines. Methods: Four different cell lines (MET 5A/normal mesothelium; H2052/sarcomatoid mesothelioma; ISTMES2/epithelial mesothelioma; MSTO/biphasic mesothelioma) were incubated with increasing concentrations of diluted PVP-I (0.0001; 0.001; 0.01; 0.1; 1{\%}) for 5, 10, 30, 60 min and 24 h, respectively. Cell viability was determined using cell direct cytotoxicity assay and cell death was determined through flow cytometry assay analysis. The superoxide dismutase activity was assessed functionally through a specific inhibitor to evaluate the mechanism of cell death. Results: The antiproliferative effect of PVP-I varied largely among different cell lines in a dose- and time-dependent manner. At 0.1{\%} concentration for 10 min of incubation, the percentage of viable cells was 0.5 ± 0.1; 0.8 ± 0.5 and 0{\%} (P <0.01) for MET5A, ISTMES2 and MSTO, respectively. Conversely, the same concentration did not significantly affect the H2052 cell line which was completely suppressed at a 1{\%} concentration of PVP-I. Double staining of Annexin V and DNA showed that PVP-I induced cell death in all four cell lines via necrosis depending on PVP-I concentration. However, H2052 was found to be more resistant than MSTO, ISTMES2 and MET 5A cells lines. The activity of superoxide dismutase was significantly inhibited in all cell lines. Conclusions: Our results confirmed the anti-neoplastic activity of PVP-I especially on ISTMES2 and MSTO cell lines. With respect to chemotherapy pleural irrigation, washing with PVP-I is cost-effective and easy. If confirmed by larger studies, our findings suggest that the intrapleural irrigation with PVP-I (0.1{\%} concentration for 10 min) in patients with epithelial or biphasic mesothelioma undergoing cytoreductive surgery might be applied in thoracic surgery practice to prevent neoplastic cell growth.",
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author = "Alfonso Fiorelli and Francesca Pentimalli and Vittorio D'urso and {Di Marzo}, Domenico and Forte, {Iris Maria} and Antonio Giordano and {Di Domenico}, Marina and Marina Accardo and {Di Serio}, Umberto and Mario Santini",
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T2 - Results of in vitro study

AU - Fiorelli, Alfonso

AU - Pentimalli, Francesca

AU - D'urso, Vittorio

AU - Di Marzo, Domenico

AU - Forte, Iris Maria

AU - Giordano, Antonio

AU - Di Domenico, Marina

AU - Accardo, Marina

AU - Di Serio, Umberto

AU - Santini, Mario

PY - 2014

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N2 - Objective: Povidone-iodine (PVP-I) or Betadine, owing to its antineoplastic activity, is also used as an adjuvant during intra-abdominal or intrathoracic surgery. However, the protocol of PVP-I administration has not been optimized to achieve the best antitumoural efficacy. We aimed to determine the optimal concentration of PVP-I, the time of incubation and the mechanism of cell death by analysing the effect of different doses and time of administration of PVP-I on the cell viability of different mesothelioma cell lines. Methods: Four different cell lines (MET 5A/normal mesothelium; H2052/sarcomatoid mesothelioma; ISTMES2/epithelial mesothelioma; MSTO/biphasic mesothelioma) were incubated with increasing concentrations of diluted PVP-I (0.0001; 0.001; 0.01; 0.1; 1%) for 5, 10, 30, 60 min and 24 h, respectively. Cell viability was determined using cell direct cytotoxicity assay and cell death was determined through flow cytometry assay analysis. The superoxide dismutase activity was assessed functionally through a specific inhibitor to evaluate the mechanism of cell death. Results: The antiproliferative effect of PVP-I varied largely among different cell lines in a dose- and time-dependent manner. At 0.1% concentration for 10 min of incubation, the percentage of viable cells was 0.5 ± 0.1; 0.8 ± 0.5 and 0% (P <0.01) for MET5A, ISTMES2 and MSTO, respectively. Conversely, the same concentration did not significantly affect the H2052 cell line which was completely suppressed at a 1% concentration of PVP-I. Double staining of Annexin V and DNA showed that PVP-I induced cell death in all four cell lines via necrosis depending on PVP-I concentration. However, H2052 was found to be more resistant than MSTO, ISTMES2 and MET 5A cells lines. The activity of superoxide dismutase was significantly inhibited in all cell lines. Conclusions: Our results confirmed the anti-neoplastic activity of PVP-I especially on ISTMES2 and MSTO cell lines. With respect to chemotherapy pleural irrigation, washing with PVP-I is cost-effective and easy. If confirmed by larger studies, our findings suggest that the intrapleural irrigation with PVP-I (0.1% concentration for 10 min) in patients with epithelial or biphasic mesothelioma undergoing cytoreductive surgery might be applied in thoracic surgery practice to prevent neoplastic cell growth.

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