Antiovarian carcinoma anti-T3 heteroconjugates or hybrid antibodies induce tumor cell lysis by cytotoxic T-cells

S. Canevari, S. Menard, D. Mezzanzanica, S. Miotti, S. M. Pupa, A. Lanzavecchia, M. I. Colnaghi

Research output: Contribution to journalArticle

Abstract

In the perspective of therapeutic in vivo targeting for T-cell attack, the monoclonal antibody (MAb) MOv18, selected for its restricted reactivity with human ovarian carcinoma, and an anti-T3 MAb were used to produce heteroconjugate or hybrid antibodies derived by fusion of relevant hybridomas. Specificity and activity of bispecific MAbs were analyzed by solid-phase RIA, immunofluorescence and a 51Cr-release assay on the ovarian carcinoma cell line OVCA 432, which expresses the relevant tumor-associated antigen, and on several irrelevant tumor cell lines. Both reagents efficiently promoted, at picomolar concentration, target cell lysis by cytotoxic T-cell (CTL) clones. Although the pattern of tumor cell lines which were lysed was wider than that predicted by binding studies, further studies using a double-determinant immunoradiometric assay confirmed the specificity of MAb targeting. Analysis of reagents indicated that the hybrid MAb was superior to the heteroaggregate as far as purification recovery and storage stability were concerned. Besides CTL clones, peripheral blood lymphocytes could also be used as cytolytic effectors, provided that a suitable in vitro activation scheme was used.

Original languageEnglish
Pages (from-to)18-21
Number of pages4
JournalInternational Journal of Cancer
Volume41
Issue numberSUPPL. 2
Publication statusPublished - 1988

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Neoplasm Antibodies
Monoclonal Antibodies
Tumor Cell Line
Carcinoma
T-Lymphocytes
Clone Cells
Immunoradiometric Assay
Hybridomas
Neoplasm Antigens
Fluorescent Antibody Technique
Lymphocytes
Cell Line
Antibodies
Therapeutics

ASJC Scopus subject areas

  • Cancer Research
  • Medicine(all)
  • Oncology

Cite this

Antiovarian carcinoma anti-T3 heteroconjugates or hybrid antibodies induce tumor cell lysis by cytotoxic T-cells. / Canevari, S.; Menard, S.; Mezzanzanica, D.; Miotti, S.; Pupa, S. M.; Lanzavecchia, A.; Colnaghi, M. I.

In: International Journal of Cancer, Vol. 41, No. SUPPL. 2, 1988, p. 18-21.

Research output: Contribution to journalArticle

Canevari, S. ; Menard, S. ; Mezzanzanica, D. ; Miotti, S. ; Pupa, S. M. ; Lanzavecchia, A. ; Colnaghi, M. I. / Antiovarian carcinoma anti-T3 heteroconjugates or hybrid antibodies induce tumor cell lysis by cytotoxic T-cells. In: International Journal of Cancer. 1988 ; Vol. 41, No. SUPPL. 2. pp. 18-21.
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AU - Menard, S.

AU - Mezzanzanica, D.

AU - Miotti, S.

AU - Pupa, S. M.

AU - Lanzavecchia, A.

AU - Colnaghi, M. I.

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