TY - JOUR
T1 - Antioxidant enzymatic activities and lipid peroxidation in cultured human chondrocytes from vertebral plate cartilage
AU - Grazioli, Vittorio
AU - Schiavo, Roberta
AU - Casari, Erminia
AU - Marzatico, Fulvio
AU - Rodriguez Y Baena, Riccardo
AU - Gaetani, Paolo
PY - 1998/7/17
Y1 - 1998/7/17
N2 - In vitro models based on primary cultured human chondrocytes could be useful to study the ROS-mediated inflammatory processes that seem to involve chondrocytes in vivo. In this work, we studied the enzymatic antioxidative capability of human chondrocytes removed from vertebral plates during micro-discectomy nod cultured 18 days, measuring total superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSHPx) activities. We also evaluated in the same cells the amount of malondialdehyde (MDA) in order to verify the effect of the variation of the cellular enzymatic antioxidative capability on the degree of membrane lipid peroxidation. Total SOD activity increased, even if not significantly, between the 12th and the 18th day. A significant variation of GSHPx (P <0.01) and of catalase (P <0.001) activity was observed between the 3rd and the 6th day with no further variation until the 18th day. A significant increase (P <0.001) of lipid peroxidation from the 3rd to the 18th day was also observed. These results seem to indicate that only fresh human cultured chondrocytes are suitable to study, through in vitro models, the in vivo behavior of the antioxidative status of these cells.
AB - In vitro models based on primary cultured human chondrocytes could be useful to study the ROS-mediated inflammatory processes that seem to involve chondrocytes in vivo. In this work, we studied the enzymatic antioxidative capability of human chondrocytes removed from vertebral plates during micro-discectomy nod cultured 18 days, measuring total superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSHPx) activities. We also evaluated in the same cells the amount of malondialdehyde (MDA) in order to verify the effect of the variation of the cellular enzymatic antioxidative capability on the degree of membrane lipid peroxidation. Total SOD activity increased, even if not significantly, between the 12th and the 18th day. A significant variation of GSHPx (P <0.01) and of catalase (P <0.001) activity was observed between the 3rd and the 6th day with no further variation until the 18th day. A significant increase (P <0.001) of lipid peroxidation from the 3rd to the 18th day was also observed. These results seem to indicate that only fresh human cultured chondrocytes are suitable to study, through in vitro models, the in vivo behavior of the antioxidative status of these cells.
KW - Catalase
KW - Glutathione peroxidase
KW - Human chondrocyte
KW - ROS
KW - Superoxide dismutase
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UR - http://www.scopus.com/inward/citedby.url?scp=0032541082&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(98)00731-5
DO - 10.1016/S0014-5793(98)00731-5
M3 - Article
C2 - 9708892
AN - SCOPUS:0032541082
VL - 431
SP - 149
EP - 153
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2
ER -