Antiphospholipid antibodies affect human endometrial angiogenesis

Nicoletta Di Simone, Fiorella Di Nicuolo, Silvia D'Ippolito, Roberta Castellani, Chiara Tersigni, Alessandro Caruso, Pierluigi Meroni, Riccardo Marana

Research output: Contribution to journalArticle

Abstract

Antiphospholipid antibodies (aPL) represent an important risk factor for thrombosis and recurrent miscarriage in patients with antiphospholipid syndrome (APS). The mechanisms of aPL-mediated pregnancy failure have been researched. Previous studies demonstrated that aPL bind trophoblast cells, reducing proliferation, human chorionic gonadotrophin release, and in vitro invasiveness. Recent data suggest that aPL are also able to react with human decidual cells, inducing a proinflammatory phenotype. Decidua, a newly formed tissue on the maternal side of the human placenta, is characterized by active angiogenesis and structural modifications of the spiral arteries in early pregnancy. Since angiogenesis is a critical component of normal placentation, the purpose of our study was to evaluate the role of aPL on human endometrial angiogenesis. For this reason, we investigated the effect of aPL on in vitro endometrial endothelial cell (HEEC) angiogenesis, VEGF secretion by ELISA, matrix metalloproteinases (MMPs) activity by gelatin zymography, and DNA binding activity of NFKB by a sensitive multiwell colorimetric assay. Furthermore, we performed experiments to study whether aPL affects in vivo angiogenesis in a murine model. We found that aPL significantly decrease the number and the total length of the tubules formed by HEEC on in vitro Matrigel assay and reduce newly formed vessels in aPL-inoculated mice. Moreover, aPL reduce significantly both VEGF and MMPs production and, at the nuclear level, NFKB DNA binding activity. From our results, it appears that aPL are associated with an inhibition of angiogenesis, suggesting further additional mechanisms to explain the defective placentation in the APS.

Original languageEnglish
Pages (from-to)212-219
Number of pages8
JournalBiology of Reproduction
Volume83
Issue number2
DOIs
Publication statusPublished - 2010

Fingerprint

Antiphospholipid Antibodies
Placentation
Antiphospholipid Syndrome
Matrix Metalloproteinases
Vascular Endothelial Growth Factor A
Decidua
Habitual Abortion
Pregnancy
DNA
Trophoblasts
Chorionic Gonadotropin
Gelatin
Placenta
Thrombosis
Endothelial Cells
Arteries
Enzyme-Linked Immunosorbent Assay
Mothers
Cell Proliferation

Keywords

  • Angiogenesis
  • Antiphospholipid syndrome
  • Endometrial endothelium
  • NFKB
  • Placenta

ASJC Scopus subject areas

  • Cell Biology
  • Medicine(all)

Cite this

Di Simone, N., Di Nicuolo, F., D'Ippolito, S., Castellani, R., Tersigni, C., Caruso, A., ... Marana, R. (2010). Antiphospholipid antibodies affect human endometrial angiogenesis. Biology of Reproduction, 83(2), 212-219. https://doi.org/10.1095/biolreprod.110.083410

Antiphospholipid antibodies affect human endometrial angiogenesis. / Di Simone, Nicoletta; Di Nicuolo, Fiorella; D'Ippolito, Silvia; Castellani, Roberta; Tersigni, Chiara; Caruso, Alessandro; Meroni, Pierluigi; Marana, Riccardo.

In: Biology of Reproduction, Vol. 83, No. 2, 2010, p. 212-219.

Research output: Contribution to journalArticle

Di Simone, N, Di Nicuolo, F, D'Ippolito, S, Castellani, R, Tersigni, C, Caruso, A, Meroni, P & Marana, R 2010, 'Antiphospholipid antibodies affect human endometrial angiogenesis', Biology of Reproduction, vol. 83, no. 2, pp. 212-219. https://doi.org/10.1095/biolreprod.110.083410
Di Simone N, Di Nicuolo F, D'Ippolito S, Castellani R, Tersigni C, Caruso A et al. Antiphospholipid antibodies affect human endometrial angiogenesis. Biology of Reproduction. 2010;83(2):212-219. https://doi.org/10.1095/biolreprod.110.083410
Di Simone, Nicoletta ; Di Nicuolo, Fiorella ; D'Ippolito, Silvia ; Castellani, Roberta ; Tersigni, Chiara ; Caruso, Alessandro ; Meroni, Pierluigi ; Marana, Riccardo. / Antiphospholipid antibodies affect human endometrial angiogenesis. In: Biology of Reproduction. 2010 ; Vol. 83, No. 2. pp. 212-219.
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