Antiproliferative and proapoptotic effects of raloxifene on uterine leiomyomas in postmenopausal women

Stefano Palomba, Francesco Orio, Tiziana Russo, Angela Falbo, Achille Tolino, Gaetano Lombardi, Vincenzo Cimini, Fulvio Zullo

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To study the cell effects of raloxifene on uterine and leiomyoma tissue in postmenopausal women. Design: Prospective, randomized, double-blind, placebo-controlled study. Setting: Department of Obstetrics and Gynecology, University "Magna Graecia" of Catanzaro, Italy. Patient(s): Forty postmenopausal women affected by uterine leiomyomas and selected for hysterectomy. Intervention(s): Treatment for three cycles of 28 days with raloxifene at a dose of 180 mg/day orally (raloxifene group) or placebo tablets (3 tablets/day orally) (placebo group). Main Outcome Measure(s): Uterine and leiomyoma dimensions were measured in each subject at entry and before surgery. On leiomyomas and homologous myometrium the proliferating cell nuclear antigen (PCNA)-positive cells/total cells (PCNA/TC) and the Bcl-2-positive cells/Bax-positive cells (Bcl-2/Bax) ratios (%), as proliferation and apoptotic indexes, respectively, were measured. Result(s): After treatment, uterine and leiomyoma sizes were significantly changed in comparison with baseline and the placebo group. PCNA/TC and Bcl-2/Bax ratios were significantly higher in leiomyomas than in homologous myometrium. A significant difference was detected in PCNA/TC between the myometrium of the raloxifene and control groups, whereas no difference was observed in the Bcl-2/Bax ratio. A significant difference in PCNA/TC and Bcl-2/Bax ratios was detected in leiomyoma tissue between the raloxifene group and controls. Conclusion(s): In postmenopausal women, raloxifene administration reduces uterine leiomyomas by exerting a cell antiproliferative and proapoptotic action.

Original languageEnglish
Pages (from-to)154-161
Number of pages8
JournalFertility and Sterility
Volume84
Issue number1
DOIs
Publication statusPublished - Jul 2005

Keywords

  • Apoptosis
  • Bax
  • Bcl-2
  • Leiomyoma
  • PCNA
  • Raloxifene
  • SERM

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

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