Antiproliferative effect of Aurora kinase targeting in mesothelioma

Stefania Crispi; Claudia Fagliarone; Annamaria Biroccio; Carmen D'Angelo; Rossella Galati; Ada Sacchi; Bruno Vincenzi; Alfonso Baldi; Alessandra Verdina

doi:10.1016/j.lungcan.2010.03.005

Antiproliferative effect of Aurora kinase targeting in mesothelioma

Stefania Crispi, Claudia Fagliarone, Annamaria Biroccio, Carmen D'Angelo, Rossella Galati, Ada Sacchi, Bruno Vincenzi, Alfonso Baldi, Alessandra Verdina

Istituto Regina Elena

Research output: Contribution to journal › Article › peer-review

Abstract

The Aurora proteins are a small family of serine/threonine kinase that function in various stages of mitosis. Current interest in Aurora kinase relates to its role in tumours, and its potential as a therapeutic target. In this work we studied the expression of Aurora kinases A and B and related genes in human mesothelioma tissues and in five mesothelioma cell lines. Moreover, we analyzed the effects of ZM447439 (ZM), an Aurora kinase inhibitor, on cellular growth. Results evidenced an over-expression of Aurora kinase A and related genes in human mesothelioma tissues and an over-expression of Aurora kinases A and B in all cell lines. Moreover, we demonstrated that ZM447439 was able to inhibit cell growth in all cell lines and that this inhibition was due to a specific effect as demonstrated by the reduction in the level of Histone H3 phosphorylation. Our findings support a role of Aurora kinase in mesothelioma and the possibility of using Aurora kinase inhibitors in therapeutic modalities.

Original language	English
Pages (from-to)	271-279
Number of pages	9
Journal	Lung Cancer
Volume	70
Issue number	3
DOIs	https://doi.org/10.1016/j.lungcan.2010.03.005
Publication status	Published - Dec 2010

Keywords

Aurora kinase
Inhibitor
Mesothelioma
Potential target
Related genes
ZM447439

ASJC Scopus subject areas

Oncology
Pulmonary and Respiratory Medicine
Cancer Research

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10.1016/j.lungcan.2010.03.005

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abstract = "The Aurora proteins are a small family of serine/threonine kinase that function in various stages of mitosis. Current interest in Aurora kinase relates to its role in tumours, and its potential as a therapeutic target. In this work we studied the expression of Aurora kinases A and B and related genes in human mesothelioma tissues and in five mesothelioma cell lines. Moreover, we analyzed the effects of ZM447439 (ZM), an Aurora kinase inhibitor, on cellular growth. Results evidenced an over-expression of Aurora kinase A and related genes in human mesothelioma tissues and an over-expression of Aurora kinases A and B in all cell lines. Moreover, we demonstrated that ZM447439 was able to inhibit cell growth in all cell lines and that this inhibition was due to a specific effect as demonstrated by the reduction in the level of Histone H3 phosphorylation. Our findings support a role of Aurora kinase in mesothelioma and the possibility of using Aurora kinase inhibitors in therapeutic modalities.",

keywords = "Aurora kinase, Inhibitor, Mesothelioma, Potential target, Related genes, ZM447439",

author = "Stefania Crispi and Claudia Fagliarone and Annamaria Biroccio and Carmen D'Angelo and Rossella Galati and Ada Sacchi and Bruno Vincenzi and Alfonso Baldi and Alessandra Verdina",

year = "2010",

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doi = "10.1016/j.lungcan.2010.03.005",

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AU - Crispi, Stefania

AU - Fagliarone, Claudia

AU - Biroccio, Annamaria

AU - D'Angelo, Carmen

AU - Galati, Rossella

AU - Sacchi, Ada

AU - Vincenzi, Bruno

AU - Baldi, Alfonso

AU - Verdina, Alessandra

PY - 2010/12

Y1 - 2010/12

N2 - The Aurora proteins are a small family of serine/threonine kinase that function in various stages of mitosis. Current interest in Aurora kinase relates to its role in tumours, and its potential as a therapeutic target. In this work we studied the expression of Aurora kinases A and B and related genes in human mesothelioma tissues and in five mesothelioma cell lines. Moreover, we analyzed the effects of ZM447439 (ZM), an Aurora kinase inhibitor, on cellular growth. Results evidenced an over-expression of Aurora kinase A and related genes in human mesothelioma tissues and an over-expression of Aurora kinases A and B in all cell lines. Moreover, we demonstrated that ZM447439 was able to inhibit cell growth in all cell lines and that this inhibition was due to a specific effect as demonstrated by the reduction in the level of Histone H3 phosphorylation. Our findings support a role of Aurora kinase in mesothelioma and the possibility of using Aurora kinase inhibitors in therapeutic modalities.

AB - The Aurora proteins are a small family of serine/threonine kinase that function in various stages of mitosis. Current interest in Aurora kinase relates to its role in tumours, and its potential as a therapeutic target. In this work we studied the expression of Aurora kinases A and B and related genes in human mesothelioma tissues and in five mesothelioma cell lines. Moreover, we analyzed the effects of ZM447439 (ZM), an Aurora kinase inhibitor, on cellular growth. Results evidenced an over-expression of Aurora kinase A and related genes in human mesothelioma tissues and an over-expression of Aurora kinases A and B in all cell lines. Moreover, we demonstrated that ZM447439 was able to inhibit cell growth in all cell lines and that this inhibition was due to a specific effect as demonstrated by the reduction in the level of Histone H3 phosphorylation. Our findings support a role of Aurora kinase in mesothelioma and the possibility of using Aurora kinase inhibitors in therapeutic modalities.

KW - Aurora kinase

KW - Inhibitor

KW - Mesothelioma

KW - Potential target

KW - Related genes

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JO - Lung Cancer

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Antiproliferative effect of Aurora kinase targeting in mesothelioma

Abstract

Keywords

ASJC Scopus subject areas

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