Antithrombin III trento: A 'new' congenital at III abnormality with a peculiar: Crossed-immunoelectrophoretic pattern in the absence of heparin

A. Girolami, F. Marafioti, M. Rubertelli, M. A. Vicarioto, G. Cappellato, M. Mazzuccato

Research output: Contribution to journalArticlepeer-review

Abstract

A family with a new congenital abnormality of antithrombin III (AT III) is presented. 5 members, all females, were affected. The proposita has had several throm-botic manifestations. The other patients, so far, are asymptomatic. Antithrombin activities were all decreased regardless of the method used (chromogenic or clotting) and regardless of the presence or absence of heparin in the assay system. AT 111 antigen, on the contrary, was normal in all patients regardless of the method used (electroimmunoas-say, radial immunodiffusion or Laser nephelometer). The crossed immunoelectrophore-sis without heparin showed in plasma the presence of an abnormal peak which was more anodal than the normal counterpart. The same pattern was seen in serum. In the heparin-modified cross-immunoelectrophoresis a normal pattern was seen in plasma and an abnormal one in serum. In the latter the anodal peak was in fact larger than the normal counterpart. Chromatographic studies using Heparin-Sepharose column failed to show changes in heparin affinity, and indicated that both the normal and the abnormal peak were contained in the major AT III area. The toponym AT III Trento is proposed to describe this abnormality. These studies further emphasize the great heterogeneity of AT III defects. This is the first AT III abnormality to show an abnormal crossed-immunoelectrophoresis in the absence of heparin.

Original languageEnglish
Pages (from-to)73-82
Number of pages10
JournalActa Haematologica
Volume72
Issue number2
Publication statusPublished - 1984

Keywords

  • Antithrombin III abnormality
  • Heparin
  • Thrombosis

ASJC Scopus subject areas

  • Hematology

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