Antithrombotic properties of dermatan sulphate in a rat venous thrombosis model

Antonio Maggi, Marzia Abbadini, P. G. Pagella, Anna Borowska, Jerta Pangrazzi, Maria Benedetta Donatia

Research output: Contribution to journalArticlepeer-review

Abstract

It has been suggested that glycosaminoglycans (GAG) such as heparan sulphate (HS), dermatan sulphate (DS), chondroitin-4-sulphate and chondroitin-6-sulphate contribute to the nonthrombogenic properties of the vascular wall [ 1 ]. We have investigated the potential role of DS and HS as antithrombotic agents in an experimental model of stasis-induced venous thrombosis in rats. We utilized a range of doses of both DS and HS (0.25-4 mg/kg BW) to test both their antithrombotic activity and potential bleeding effects. The results were evaluated with reference to an unfractionated heparin (0.5-2 mg/kg BW). We report that the antithrombotic activity of DS is not related to its anticoagulant activity as measured by the activated partial thromboplastin time (APTT), thrombin time (TT) and anti-Xa tests. The dose of DS which was able to inhibit thrombus formation by 70% did not prolong the bleeding time measured using two techniques (template and tail transection); in contrast, with HS a prolongation of both times could clearly be seen. On the other hand, standard unfractionated heparin, at a dose which is equipotent to that of DS in preventing thrombus formation, significantly prolonged the bleeding time. These results suggest that DS may be a useful antithrombotic agent with a lower haemorrhagic effect than heparin, unlike HS which expresses a haemorrhagic risk similar to heparin.

Original languageEnglish
Pages (from-to)329-335
Number of pages7
JournalPathophysiology of Haemostasis and Thrombosis
Volume17
Issue number6
DOIs
Publication statusPublished - 1987

Keywords

  • Antithrombotic activity
  • Dermatan sulphate
  • Haemorrhage

ASJC Scopus subject areas

  • Hematology
  • Physiology (medical)

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