Antitumor activity of interferon-β1a in hormone refractory prostate cancer with neuroendocrine differentiation

A. Dicitore, E. S. Grassi, M. O. Borghi, G. Gelmini, M. C. Cantone, G. Gaudenzi, L. Persani, M. Caraglia, G. Vitale

Research output: Contribution to journalArticle

Abstract

Purpose: Type I interferons (IFN-α and IFN-β) are a class of cytokines that exert several biological activities, such as modulation of cell proliferation and differentiation and of the immune system. Although these cytokines interact with a common receptor complex, IFN-β showed a more potent antitumor activity than IFN-α in several tumor models. New recombinant human IFN-β products, such as IFN-β1a and IFN-β1b, have been produced in order to improve the stability and bioavailability of natural IFN-β. In this report, we analyzed the effects of recombinant IFN-β1a on the cell proliferation of two human androgen-resistant prostate cancer cell lines with neuroendocrine differentiation (DU-145, PC-3) and related mechanisms of action. Methods: The effects of IFN-β1a on the cell growth proliferation, cell cycle, and apoptosis have been evaluated in DU-145 and PC-3 cells through MTT assay, DNA flow cytometry with propidium iodide, and Annexin V-FITC/propidium iodide staining, respectively. Moreover, the expression of neuron-specific enolase (NSE), cleaved caspase-3, caspase-8, and PARP was evaluated through Western blotting. Results: IFN-β1a showed a significant anti-proliferative activity in both androgen-resistant cell lines. This effect was related to cell cycle perturbation and induction in apoptosis, as shown by flow cytometric analysis, the activation of caspase-3 and caspase-8 and PARP cleavage during incubation with IFN-β1a. Moreover, this cytokine reduced the expression of NSE in both cell lines. Conclusions: Recombinant IFN-β1a (Rebif) showed a potent in vitro anti-proliferative activity in androgen-resistant prostate cancer cells, and it could represent a promising tool for the treatment of this tumor.

Original languageEnglish
Pages (from-to)761-770
Number of pages10
JournalJournal of Endocrinological Investigation
Volume40
Issue number7
DOIs
Publication statusPublished - Jul 1 2017

Keywords

  • Apoptosis
  • Castration-resistant prostate cancer
  • Cell cycle
  • Interferon beta
  • Type I interferons

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Fingerprint Dive into the research topics of 'Antitumor activity of interferon-β1a in hormone refractory prostate cancer with neuroendocrine differentiation'. Together they form a unique fingerprint.

  • Cite this