Antitumor effect of green tea polyphenol epigallocatechin-3-gallate in ovarian carcinoma cells: Evidence for the endothelin-1 as a potential target

Francesca Spinella, Laura Rosanò, Samantha Decandia, Valeriana Di Castro, Adriana Albini, Giacomo Elia, Pier Giorgio Natali, Anna Bagnato

Research output: Contribution to journalArticlepeer-review

Abstract

The green tea polyphenol, epigallocatechin-3-gallate (EGCG), has been shown to prevent cancer; however, a precise mechanism responsible for tumor growth inhibition has not yet been clearly described. The endothelin (ET) A receptor (ETAR)/ET-1 autocrine pathway is overexpressed in ovarian carcinoma and triggers tumor growth, neoangiogenesis, and invasion. These latter tumor-promoting effects are mediated through the activation of cyclooxygenase (COX)-1- and COX-2-dependent pathways by ET-1. In the present study, pretreatment of HEY and OVCA 433 ovarian carcinoma cell lines with green tea and EGCG inhibited ET-1/ETAR expression, ETAR-mediated COX-1/2 mRNA expression, and COX-2 promoter activity. These effects were associated with a significant reduction in the COX-1/2-derived prostaglandin E2 (PGE2) production. These results provide a novel insight into the mechanism by which EGCG, by affecting ETAR-dependent COX-1/2 pathways may inhibit ovarian tumors suggesting that EGCG may be useful in preventing and treating ovarian carcinoma in which activation of ET AR by ET-1 plays a critical role in tumor growth and progression.

Original languageEnglish
Pages (from-to)1123-1127
Number of pages5
JournalExperimental Biology and Medicine
Volume231
Issue number6
Publication statusPublished - Jun 2006

Keywords

  • EGCG
  • Endothelin-1
  • ET receptor
  • Green tea
  • Ovarian carcinoma

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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