Aortic valve cell seeding into decellularized animal pericardium by perfusion-assisted bioreactor

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Animal-derived pericardium is the elective tissue employed in manufacturing heart valve prostheses. The preparation of this tissue for biological valve production consists of fixation with aldehydes, which reduces, but not eliminates, the xenoantigens and the donor cellular material. As a consequence, especially in patients below 65–70 years of age, the employment of valve substitutes contaning pericardium is not indicated due to progressive calcification that causes tissue degeneration and recurrence of valve insufficiency. Decellularization with ionic or nonionic detergents has been proposed as an alternative procedure to prepare aldehyde- or xenoantigen-free pericardium for biological valve manufacturing. In the present contribution, we optimized a decellularization procedure that is permissive for seeding and culturing valve competent cells able to colonize and reconstitute a valve-like tissue. A high-efficiency cellularization was achieved by forcing cell penetration inside the pericardium matrix using a perfusion bioreactor. Because the decellularization procedure was found not to alter the collagen composition of the pericardial matrix and cells seeded in the tissue constructs consistently grew and acquired the phenotype of “quiescent” valve interstitial cells, our investigation sets a novel standard in pericardium application for tissue engineering of “living” valve implants.

Original languageEnglish
Pages (from-to)1481-1493
Number of pages13
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume12
Issue number6
DOIs
Publication statusPublished - Jun 1 2018

Fingerprint

Pericardium
Bioreactors
Aortic Valve
Animals
Perfusion
Tissue
Heterophile Antigens
Aldehydes
Heart valve prostheses
Heart Valve Prosthesis
Detergents
Tissue Engineering
Tissue engineering
Collagen
Tissue Donors
Phenotype
Recurrence
Chemical analysis

Keywords

  • bioreactor
  • decellularization
  • pericardium
  • tissue-engineered heart valve
  • valve interstitial cell
  • xenoantigen

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

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title = "Aortic valve cell seeding into decellularized animal pericardium by perfusion-assisted bioreactor",
abstract = "Animal-derived pericardium is the elective tissue employed in manufacturing heart valve prostheses. The preparation of this tissue for biological valve production consists of fixation with aldehydes, which reduces, but not eliminates, the xenoantigens and the donor cellular material. As a consequence, especially in patients below 65–70 years of age, the employment of valve substitutes contaning pericardium is not indicated due to progressive calcification that causes tissue degeneration and recurrence of valve insufficiency. Decellularization with ionic or nonionic detergents has been proposed as an alternative procedure to prepare aldehyde- or xenoantigen-free pericardium for biological valve manufacturing. In the present contribution, we optimized a decellularization procedure that is permissive for seeding and culturing valve competent cells able to colonize and reconstitute a valve-like tissue. A high-efficiency cellularization was achieved by forcing cell penetration inside the pericardium matrix using a perfusion bioreactor. Because the decellularization procedure was found not to alter the collagen composition of the pericardial matrix and cells seeded in the tissue constructs consistently grew and acquired the phenotype of “quiescent” valve interstitial cells, our investigation sets a novel standard in pericardium application for tissue engineering of “living” valve implants.",
keywords = "bioreactor, decellularization, pericardium, tissue-engineered heart valve, valve interstitial cell, xenoantigen",
author = "Francesco Amadeo and Federica Boschetti and Gianluca Polvani and Cristina Banfi and Maurizio Pesce and Rosaria Santoro",
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T1 - Aortic valve cell seeding into decellularized animal pericardium by perfusion-assisted bioreactor

AU - Amadeo, Francesco

AU - Boschetti, Federica

AU - Polvani, Gianluca

AU - Banfi, Cristina

AU - Pesce, Maurizio

AU - Santoro, Rosaria

PY - 2018/6/1

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N2 - Animal-derived pericardium is the elective tissue employed in manufacturing heart valve prostheses. The preparation of this tissue for biological valve production consists of fixation with aldehydes, which reduces, but not eliminates, the xenoantigens and the donor cellular material. As a consequence, especially in patients below 65–70 years of age, the employment of valve substitutes contaning pericardium is not indicated due to progressive calcification that causes tissue degeneration and recurrence of valve insufficiency. Decellularization with ionic or nonionic detergents has been proposed as an alternative procedure to prepare aldehyde- or xenoantigen-free pericardium for biological valve manufacturing. In the present contribution, we optimized a decellularization procedure that is permissive for seeding and culturing valve competent cells able to colonize and reconstitute a valve-like tissue. A high-efficiency cellularization was achieved by forcing cell penetration inside the pericardium matrix using a perfusion bioreactor. Because the decellularization procedure was found not to alter the collagen composition of the pericardial matrix and cells seeded in the tissue constructs consistently grew and acquired the phenotype of “quiescent” valve interstitial cells, our investigation sets a novel standard in pericardium application for tissue engineering of “living” valve implants.

AB - Animal-derived pericardium is the elective tissue employed in manufacturing heart valve prostheses. The preparation of this tissue for biological valve production consists of fixation with aldehydes, which reduces, but not eliminates, the xenoantigens and the donor cellular material. As a consequence, especially in patients below 65–70 years of age, the employment of valve substitutes contaning pericardium is not indicated due to progressive calcification that causes tissue degeneration and recurrence of valve insufficiency. Decellularization with ionic or nonionic detergents has been proposed as an alternative procedure to prepare aldehyde- or xenoantigen-free pericardium for biological valve manufacturing. In the present contribution, we optimized a decellularization procedure that is permissive for seeding and culturing valve competent cells able to colonize and reconstitute a valve-like tissue. A high-efficiency cellularization was achieved by forcing cell penetration inside the pericardium matrix using a perfusion bioreactor. Because the decellularization procedure was found not to alter the collagen composition of the pericardial matrix and cells seeded in the tissue constructs consistently grew and acquired the phenotype of “quiescent” valve interstitial cells, our investigation sets a novel standard in pericardium application for tissue engineering of “living” valve implants.

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