APC10.1: An ApcMin/+ intestinal cell line with retention of heterozygosity

Carla De Giovanni, Lorena Landuzzi, Giordano Nicoletti, Annalisa Astolfi, Stefania Croci, Massimo Micaroni, Patrizia Nanni, Pier Luigi Lollini

Research output: Contribution to journalArticle

Abstract

APC10.1 is a new intestinal cell line derived from ApcMin/+ mice that retains both the heterozygous Apc genotype and a nonactivated Wnt signaling pathway and displays an early neoplastic phenotype. Although tumorigenic both in immunodepressed and in immunocompetent syngeneic mice, it requires a high cell dose and a long latency. Its epithemial/intestinal origin is shown, in a gene expression profile, by the expression of epithelial transcripts (such as cytokeratin and laminin isoforms) and of developmental regulatory genes (such as Tcf-4, Hnf3β, p21, Ihh, Hes1) necessary for, or involved in, the maintenance of intestinal stem cells. The lack of activation of the Wnt cascade in APC10.1 cell is shown both by the expression profile of Wnt target genes and by the standard TCF reporter assay. APC10.1 cell line is a novel in vitro model that can contribute to a better understanding of the clinical evolution of familial adenomatous polyposis and to finding of new prophylactic and therapeutic approaches. Supplementary material for this article can be found on the International Journal of Cancer website at http://www.inter-science.wiley.com/jpages/0020-7136/suppmat/index.html.

Original languageEnglish
Pages (from-to)200-206
Number of pages7
JournalInternational Journal of Cancer
Volume109
Issue number2
DOIs
Publication statusPublished - Mar 20 2004

Keywords

  • Adenomatous polyposis coli
  • Apc
  • Gene expression profile

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Fingerprint Dive into the research topics of 'APC10.1: An ApcMin/+ intestinal cell line with retention of heterozygosity'. Together they form a unique fingerprint.

  • Cite this

    De Giovanni, C., Landuzzi, L., Nicoletti, G., Astolfi, A., Croci, S., Micaroni, M., Nanni, P., & Lollini, P. L. (2004). APC10.1: An ApcMin/+ intestinal cell line with retention of heterozygosity. International Journal of Cancer, 109(2), 200-206. https://doi.org/10.1002/ijc.11690