Apoptosis and autoimmunity induced by clodronate in systemic lupus erythematosus mononuclear circulating cells

F. Cacciapaglia, C. Spadaccio, C. Gregorj, D. Margiotta, R. Coccia, F. De Marco, M. Chello, A. Picardi, A. Amoroso, Antonella Afeltra

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of this study is to evaluate the effect of clodronate on apoptosis of human systemic lupus erythematosus circulating mononuclear cells and to analyze possible correlations with changes in autoantibody production in vitro. Lympho-monocytes from 20 SLE patients were isolated and incubated with or without addition of 1 μM clodronate for 72 hours. Apoptosis and release of genomic material was assessed by immunofluorescent detection of cleaved caspase-3 and by Cell-Death-Detection ELISAPLUS kit (Roche). Anti-Nucleosome IgG and anti-dsDNA IgM and IgG autoantibody levels were determined in supernatants by commercially available ELISA kits. Clodronate induced apoptosis in monocytes as confirmed by cleaved caspase-3 immunostaining and by quantification of cleaved nucleosome in the supernatants (treated 0.22±0.05 O.D. vs untreated 0.09±0.04 O.D.; P

Original languageEnglish
Pages (from-to)535-542
Number of pages8
JournalInternational Journal of Immunopathology and Pharmacology
Volume23
Issue number2
Publication statusPublished - Apr 2010

Keywords

  • Anti-nucleosome antibodies
  • Apoptosis
  • Clodronate
  • Mononuclear cells
  • SLE

ASJC Scopus subject areas

  • Pharmacology
  • Immunology
  • Immunology and Allergy

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