Apoptosis and rejection in rat intestinal transplantation: Correlation with FK 506 doses and donor specific bone marrow infusions

Giovanni Vennarecci, Mariana Berho, Antonio Sommariva, Alexandre Bakonyi Neto, Evangelos P. Misiakos, Luca Inverardi, Phillip Ruiz, Camillo Ricordi, Andreas G. Tzakis

Research output: Contribution to journalArticle

Abstract

Background. Our purpose was to investigate the occurrence and the evolution of apoptosis of enterocytes during acute and chronic rejection in an experimental model of allogeneic heterotopic small bowel transplantation (SBTx). Methods. Forty-five rats were divided in 10 experimental groups according to the dose of FK506 administration and donor bone marrow infusions (DBMI). Groups 1 and 2 did not received BMI. Groups 3 and 4 received 150×106 cells at day 0, groups 5 and 6 received 75×106 cells at days 0-4, groups 7 and 8 received 75×106 cells at days 4 and 10, and groups 9 and 10 received 30×106 cells at days 4, 10, 15, 20, and 25. Animals of groups 1, 3, 5, 7, and 9 were immunosuppressed with 0.5 mg/kg FK 506, although the remaining groups with 1 mg/kg FK 506, from day 0 to 4 after transplant. Fragment end labeling of DNA was used to detect apoptosis. Results. The number of apoptotic cells detected was highest at day 15 (184±154) and then progressively decreased thereafter (day 30=159±197; day 45=80±167; day 60=0). The number of apoptotic enterocytes was found increased during mild (151±108) and moderate (281±161) allograft rejection, although a low apoptotic rate was observed in cases without rejection (59±13) and during severe (53±131) and chronic rejection (46±136). Furthermore the number of labeled cells was found inversely correlated with fibrosis (P

Original languageEnglish
Pages (from-to)1718-1724
Number of pages7
JournalTransplantation
Volume71
Issue number12
Publication statusPublished - Jun 27 2001

ASJC Scopus subject areas

  • Transplantation
  • Immunology

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    Vennarecci, G., Berho, M., Sommariva, A., Bakonyi Neto, A., Misiakos, E. P., Inverardi, L., Ruiz, P., Ricordi, C., & Tzakis, A. G. (2001). Apoptosis and rejection in rat intestinal transplantation: Correlation with FK 506 doses and donor specific bone marrow infusions. Transplantation, 71(12), 1718-1724.