Apoptosis, DNA damage and ubiquitin expression in normal and mdx muscle fibers after exercise

Marco Sandri, Ugo Carraro, Marzena Podhorska-Okolov, Corrado Rizzi, Paola Arslan, Daniela Monti, Claudio Franceschi

Research output: Contribution to journalArticle

Abstract

The current view indicates that after eccentric exercise myofibers are mechanically damaged and therefore an inflammatory and necrotic process occurs. In the present paper we examine the possibility that apoptosis plays a role in normal and dystrophin-deficient muscles after running. We analysed for apoptosis normal and dystrophin-deficient mouse muscles after a night of spontaneous wheel-running followed by two days of rest. Terminal deoxynucleotidyl transferase-mediated endlabeling of DNA in nuclei in tissue sections and gel electrophoresis of extracted DNA showed the presence of fragmented DNA. Furthermore, ubiquitin, a protein whose appearance is related to apoptosis, increased in muscles of both dystrophic and normal runner mice. The present findings which confirm that DNA damage is absent in muscles of sedentary mice but present in muscles of runner mice offer a new hypothesis on early events of muscle damage.

Original languageEnglish
Pages (from-to)291-295
Number of pages5
JournalFEBS Letters
Volume373
Issue number3
DOIs
Publication statusPublished - Oct 16 1995

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Keywords

  • Apoptosis
  • Muscle damage
  • Ubiquitin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

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