TY - JOUR
T1 - Apoptosis in asymptomatic HIV-1 seropositives immunized with HIV-1 env glycoprotein (gp160)
T2 - Effects of administration of Zidovudine in vivo and interleukin-2 in vitro
AU - Pierdominici, Marina
AU - Mollicone, Bianca
AU - Ricci, Giovanni
AU - Oliva, Alessandra
AU - D'Offizi, Gianpiero
AU - Giovannetti, Antonello
AU - Aiuti, Fernando
AU - Pandolfi, Franco
PY - 1998/4
Y1 - 1998/4
N2 - In this paper we report the effects of VaxSyn® (Protein Sciences Corp.) immunization on spontaneous apoptosis occurring in vitro after culture of PBMC in medium alone in 30 HIV-seropositive patients enrolled in a double- blind clinical trial that included three groups: treatment with VaxSyn, AZT and VaxSyn, and AZT. Our data show no significant modifications in the levels of apoptosis observed in the three groups over the long-term follow-up (up to 720 days). This was not associated with any significant modifications in other clinical or immunological features. However, analysis of apoptosis performed shortly after the first immunization (at days 3 and 7) showed a significant reduction in the rate of apoptosis in patients receiving AZT or AZT and VaxSyn, as compared with patients receiving VaxSyn alone (30.42 ± 2.52 SE at day 0 and 23.74 ± 1.84 at day 3; p = 0.039). Our data also indicate that addition of IL-2 in vitro had a significant inhibitory effect on mortality in all the randomization groups, especially in those receiving AZT (alone or in combination with VaxSyn).
AB - In this paper we report the effects of VaxSyn® (Protein Sciences Corp.) immunization on spontaneous apoptosis occurring in vitro after culture of PBMC in medium alone in 30 HIV-seropositive patients enrolled in a double- blind clinical trial that included three groups: treatment with VaxSyn, AZT and VaxSyn, and AZT. Our data show no significant modifications in the levels of apoptosis observed in the three groups over the long-term follow-up (up to 720 days). This was not associated with any significant modifications in other clinical or immunological features. However, analysis of apoptosis performed shortly after the first immunization (at days 3 and 7) showed a significant reduction in the rate of apoptosis in patients receiving AZT or AZT and VaxSyn, as compared with patients receiving VaxSyn alone (30.42 ± 2.52 SE at day 0 and 23.74 ± 1.84 at day 3; p = 0.039). Our data also indicate that addition of IL-2 in vitro had a significant inhibitory effect on mortality in all the randomization groups, especially in those receiving AZT (alone or in combination with VaxSyn).
UR - http://www.scopus.com/inward/record.url?scp=0032054054&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032054054&partnerID=8YFLogxK
U2 - 10.1016/S0264-410X(97)00251-X
DO - 10.1016/S0264-410X(97)00251-X
M3 - Article
C2 - 9562691
AN - SCOPUS:0032054054
VL - 16
SP - 715
EP - 721
JO - Vaccine
JF - Vaccine
SN - 0264-410X
IS - 7
ER -