Apoptosis in relation to CD34 antigen expression in normal and myelodysplastic bone marrow

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

An increased bone marrow (BM) apoptosis is one of the mechanisms responsible for the ineffective hematopoiesis of myelodysplastic syndromes (MDS). It is controversial whether the excessive apoptosis in myelodysplasia predominantly involves the subset of progenitor cells or of maturing cells. We investigated the degree of apoptosis in MDS BM and its differences from normal marrow in relation to CD34 antigen expression. A double-labelling technique that combined the Tdt-mediated dUTP nickend labelling (TUNEL) method with immunocytochemistry for CD34 antigen was used on BM slides of 18 MDS patients and 11 controls. The apoptotic rate (AR) appeared significantly higher in CD34-negative than in CD34-positive cell subsets both in myelodysplastic and in normal BM. When MDS and normal CD34-negative cell populations were compared, a greater AR in MDS CD34-negative cells was found, while no statistical difference in AR resulted from the comparison between MDS and normal CD34-positive cell populations. Our results suggest that in myelodysplasticas well as in normal BM the apoptotic phenomenon predominantly involves the maturing cells. The increase in apoptotic levels which can be observed in myelodysplastic compared to normal BM seems to be mainly due to an increase in apoptosis in the differentiated cell population.

Original languageEnglish
Pages (from-to)29-34
Number of pages6
JournalActa Haematologica
Volume109
Issue number1
DOIs
Publication statusPublished - 2003

Fingerprint

CD34 Antigens
Myelodysplastic Syndromes
Bone Marrow
Apoptosis
Population
Hematopoiesis
Stem Cells
Immunohistochemistry

Keywords

  • Apoptosis
  • CD34 antigen
  • Myelodysplastic syndromes
  • Stem cell

ASJC Scopus subject areas

  • Hematology

Cite this

@article{8022664fee9b4580a5961c10a1530305,
title = "Apoptosis in relation to CD34 antigen expression in normal and myelodysplastic bone marrow",
abstract = "An increased bone marrow (BM) apoptosis is one of the mechanisms responsible for the ineffective hematopoiesis of myelodysplastic syndromes (MDS). It is controversial whether the excessive apoptosis in myelodysplasia predominantly involves the subset of progenitor cells or of maturing cells. We investigated the degree of apoptosis in MDS BM and its differences from normal marrow in relation to CD34 antigen expression. A double-labelling technique that combined the Tdt-mediated dUTP nickend labelling (TUNEL) method with immunocytochemistry for CD34 antigen was used on BM slides of 18 MDS patients and 11 controls. The apoptotic rate (AR) appeared significantly higher in CD34-negative than in CD34-positive cell subsets both in myelodysplastic and in normal BM. When MDS and normal CD34-negative cell populations were compared, a greater AR in MDS CD34-negative cells was found, while no statistical difference in AR resulted from the comparison between MDS and normal CD34-positive cell populations. Our results suggest that in myelodysplasticas well as in normal BM the apoptotic phenomenon predominantly involves the maturing cells. The increase in apoptotic levels which can be observed in myelodysplastic compared to normal BM seems to be mainly due to an increase in apoptosis in the differentiated cell population.",
keywords = "Apoptosis, CD34 antigen, Myelodysplastic syndromes, Stem cell",
author = "Alessandro Pecci and Erica Travaglino and Catherine Klersy and Rosangela Invernizzi",
year = "2003",
doi = "10.1159/000067275",
language = "English",
volume = "109",
pages = "29--34",
journal = "Acta Haematologica",
issn = "0001-5792",
publisher = "S. Karger AG",
number = "1",

}

TY - JOUR

T1 - Apoptosis in relation to CD34 antigen expression in normal and myelodysplastic bone marrow

AU - Pecci, Alessandro

AU - Travaglino, Erica

AU - Klersy, Catherine

AU - Invernizzi, Rosangela

PY - 2003

Y1 - 2003

N2 - An increased bone marrow (BM) apoptosis is one of the mechanisms responsible for the ineffective hematopoiesis of myelodysplastic syndromes (MDS). It is controversial whether the excessive apoptosis in myelodysplasia predominantly involves the subset of progenitor cells or of maturing cells. We investigated the degree of apoptosis in MDS BM and its differences from normal marrow in relation to CD34 antigen expression. A double-labelling technique that combined the Tdt-mediated dUTP nickend labelling (TUNEL) method with immunocytochemistry for CD34 antigen was used on BM slides of 18 MDS patients and 11 controls. The apoptotic rate (AR) appeared significantly higher in CD34-negative than in CD34-positive cell subsets both in myelodysplastic and in normal BM. When MDS and normal CD34-negative cell populations were compared, a greater AR in MDS CD34-negative cells was found, while no statistical difference in AR resulted from the comparison between MDS and normal CD34-positive cell populations. Our results suggest that in myelodysplasticas well as in normal BM the apoptotic phenomenon predominantly involves the maturing cells. The increase in apoptotic levels which can be observed in myelodysplastic compared to normal BM seems to be mainly due to an increase in apoptosis in the differentiated cell population.

AB - An increased bone marrow (BM) apoptosis is one of the mechanisms responsible for the ineffective hematopoiesis of myelodysplastic syndromes (MDS). It is controversial whether the excessive apoptosis in myelodysplasia predominantly involves the subset of progenitor cells or of maturing cells. We investigated the degree of apoptosis in MDS BM and its differences from normal marrow in relation to CD34 antigen expression. A double-labelling technique that combined the Tdt-mediated dUTP nickend labelling (TUNEL) method with immunocytochemistry for CD34 antigen was used on BM slides of 18 MDS patients and 11 controls. The apoptotic rate (AR) appeared significantly higher in CD34-negative than in CD34-positive cell subsets both in myelodysplastic and in normal BM. When MDS and normal CD34-negative cell populations were compared, a greater AR in MDS CD34-negative cells was found, while no statistical difference in AR resulted from the comparison between MDS and normal CD34-positive cell populations. Our results suggest that in myelodysplasticas well as in normal BM the apoptotic phenomenon predominantly involves the maturing cells. The increase in apoptotic levels which can be observed in myelodysplastic compared to normal BM seems to be mainly due to an increase in apoptosis in the differentiated cell population.

KW - Apoptosis

KW - CD34 antigen

KW - Myelodysplastic syndromes

KW - Stem cell

UR - http://www.scopus.com/inward/record.url?scp=0037209066&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037209066&partnerID=8YFLogxK

U2 - 10.1159/000067275

DO - 10.1159/000067275

M3 - Article

C2 - 12486320

AN - SCOPUS:0037209066

VL - 109

SP - 29

EP - 34

JO - Acta Haematologica

JF - Acta Haematologica

SN - 0001-5792

IS - 1

ER -