Abstract
We report that apoptosis induced by N-hexanoylsphingosine (C6-Cer)in CHP-100 human neuroepithelioma cells associates with accumulation of monohexosylsphingolipids produced not only by short-chain ceramide glycosylation but also through glycosylation of a ceramide pool endogenously produced. By high-performance thin layer chromatography on berate silica gel plates, newly formed monohexosylsphingolipids were identified as glucosylceramides (GluCer); however, accumulation of lactosylceramide or higher-order glycosphingolipids was not observed. GluCer accumulation was fully suppressed by D-threo-1- phenyl-2-decanoylamino-3-morpholino-1-propanol; moreover, while this inhibitor had no effect on cell viability when administered alone, it markedly potentiated the apoptotic effect of C6-Cer. These results provide evidence that activation of GluCer synthesis is an important mechanism through which CHP-100 cells attempt to escape ceramide-induced apoptosis.
| Original language | English |
|---|---|
| Pages (from-to) | 785-791 |
| Number of pages | 7 |
| Journal | Cell Death and Differentiation |
| Volume | 5 |
| Issue number | 9 |
| Publication status | Published - Sep 1998 |
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Keywords
- Apoptosis
- Ceramide
- CHP-100
- Glucosylceramide
- Neuroepithelioma cells
ASJC Scopus subject areas
- Cell Biology
Cite this
Apoptosis induced by N-hexanoylsphingosine in CHP-100 cells associates with accumulation of endogenous ceramide and is potentiated by inhibition of glucocerebroside synthesis. / Spinedi, Angelo; Di Bartolomeo, Sabrina; Piacentini, Mauro.
In: Cell Death and Differentiation, Vol. 5, No. 9, 09.1998, p. 785-791.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Apoptosis induced by N-hexanoylsphingosine in CHP-100 cells associates with accumulation of endogenous ceramide and is potentiated by inhibition of glucocerebroside synthesis
AU - Spinedi, Angelo
AU - Di Bartolomeo, Sabrina
AU - Piacentini, Mauro
PY - 1998/9
Y1 - 1998/9
N2 - We report that apoptosis induced by N-hexanoylsphingosine (C6-Cer)in CHP-100 human neuroepithelioma cells associates with accumulation of monohexosylsphingolipids produced not only by short-chain ceramide glycosylation but also through glycosylation of a ceramide pool endogenously produced. By high-performance thin layer chromatography on berate silica gel plates, newly formed monohexosylsphingolipids were identified as glucosylceramides (GluCer); however, accumulation of lactosylceramide or higher-order glycosphingolipids was not observed. GluCer accumulation was fully suppressed by D-threo-1- phenyl-2-decanoylamino-3-morpholino-1-propanol; moreover, while this inhibitor had no effect on cell viability when administered alone, it markedly potentiated the apoptotic effect of C6-Cer. These results provide evidence that activation of GluCer synthesis is an important mechanism through which CHP-100 cells attempt to escape ceramide-induced apoptosis.
AB - We report that apoptosis induced by N-hexanoylsphingosine (C6-Cer)in CHP-100 human neuroepithelioma cells associates with accumulation of monohexosylsphingolipids produced not only by short-chain ceramide glycosylation but also through glycosylation of a ceramide pool endogenously produced. By high-performance thin layer chromatography on berate silica gel plates, newly formed monohexosylsphingolipids were identified as glucosylceramides (GluCer); however, accumulation of lactosylceramide or higher-order glycosphingolipids was not observed. GluCer accumulation was fully suppressed by D-threo-1- phenyl-2-decanoylamino-3-morpholino-1-propanol; moreover, while this inhibitor had no effect on cell viability when administered alone, it markedly potentiated the apoptotic effect of C6-Cer. These results provide evidence that activation of GluCer synthesis is an important mechanism through which CHP-100 cells attempt to escape ceramide-induced apoptosis.
KW - Apoptosis
KW - Ceramide
KW - CHP-100
KW - Glucosylceramide
KW - Neuroepithelioma cells
UR - http://www.scopus.com/inward/record.url?scp=0031787361&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031787361&partnerID=8YFLogxK
M3 - Article
C2 - 10200538
AN - SCOPUS:0031787361
VL - 5
SP - 785
EP - 791
JO - Cell Death and Differentiation
JF - Cell Death and Differentiation
SN - 1350-9047
IS - 9
ER -