Apoptosis induced by N-hexanoylsphingosine in CHP-100 cells associates with accumulation of endogenous ceramide and is potentiated by inhibition of glucocerebroside synthesis

Angelo Spinedi, Sabrina Di Bartolomeo, Mauro Piacentini

Research output: Contribution to journalArticlepeer-review

Abstract

We report that apoptosis induced by N-hexanoylsphingosine (C6-Cer)in CHP-100 human neuroepithelioma cells associates with accumulation of monohexosylsphingolipids produced not only by short-chain ceramide glycosylation but also through glycosylation of a ceramide pool endogenously produced. By high-performance thin layer chromatography on berate silica gel plates, newly formed monohexosylsphingolipids were identified as glucosylceramides (GluCer); however, accumulation of lactosylceramide or higher-order glycosphingolipids was not observed. GluCer accumulation was fully suppressed by D-threo-1- phenyl-2-decanoylamino-3-morpholino-1-propanol; moreover, while this inhibitor had no effect on cell viability when administered alone, it markedly potentiated the apoptotic effect of C6-Cer. These results provide evidence that activation of GluCer synthesis is an important mechanism through which CHP-100 cells attempt to escape ceramide-induced apoptosis.

Original languageEnglish
Pages (from-to)785-791
Number of pages7
JournalCell Death and Differentiation
Volume5
Issue number9
Publication statusPublished - Sep 1998

Keywords

  • Apoptosis
  • Ceramide
  • CHP-100
  • Glucosylceramide
  • Neuroepithelioma cells

ASJC Scopus subject areas

  • Cell Biology

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