Apoptosis Induced by Oxaliplatin in Human Colon Cancer HCT15 Cell Line

Paolo Marchetti, Domenico A P Galla, F. Paola Russo, Enrico Ricevuto, Vincenzo Flati, Giampiero Porzio, Corrado Ficorella, M. Grazia Cifone

Research output: Contribution to journalArticle

Abstract

Background: Oxaliplatin (L-OHP), active in a wide range of human and animal tumours, also CDDP-resistant, possesses unique molecular characteristics of action. However, the mechanisms by which the damage induced by L-OHP triggers a death signal are not yet fully defined. Materials and Methods: After L-OHP treatment of the HCT15 human colon cancer cell line, apoptosis was evaluated by DNA laddering detection and by flow cytometry; the effect on specific caspase-3, -8 and -9 inhibitors, mitochondrial membrane permeability transition, cytochrome C release and expression of CD95 and CD95L were also assessed. Results: HCT15 cells underwent apoptosis when treated with all used drug concentrations (7-25 μM). Treatment of cells with L-OHP resulted in the activation of caspase-8, -9 and -3, in a mitochondrial membrane depolarisation, and in an increase of CD95 receptor and CD95 ligand levels. Conclusion: The results correlated well with the ability of L-OHP to induce apoptosis and give further insights into the mechanisms underlying the L-OHP-induced apoptosis of tumor cells.

Original languageEnglish
Pages (from-to)219-226
Number of pages8
JournalAnticancer Research
Volume24
Issue number1
Publication statusPublished - Jan 2004

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Keywords

  • Apoptosis
  • Colon cancer cells
  • Oxaliplatin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Marchetti, P., Galla, D. A. P., Russo, F. P., Ricevuto, E., Flati, V., Porzio, G., Ficorella, C., & Cifone, M. G. (2004). Apoptosis Induced by Oxaliplatin in Human Colon Cancer HCT15 Cell Line. Anticancer Research, 24(1), 219-226.