Apoptosis induced by proteasome inhibition in human myoblast cultures

J. Sassone, Andrea Ciammola, C. Tiloca, M. Glionna, G. Meola, E. Mancinelli, V. Silani

Research output: Contribution to journalArticle

Abstract

Dysfunction of the ubiquitin-proteasome system has recently been implicated in the pathogenesis of some untreatable myodegenerative diseases characterized by the formation of ubiquitinated inclusions in skeletal muscles. We have developed an in vitro model of proteasomal dysfunction by applying inhibitors of the proteasome to primary adult human skeletal muscle cultures. Our data show that proteasome inhibition causes both cytoplasmic accumulation of ubiquitinated inclusions and apoptotic death, the latter through accumulation of active caspase-3.

Original languageEnglish
Pages (from-to)109-118
Number of pages10
JournalEuropean journal of histochemistry : EJH
Volume50
Issue number2
Publication statusPublished - Apr 2006

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Keywords

  • Aggregates
  • Apoptosis
  • Myoblasts
  • Proteasome

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy
  • Animal Science and Zoology
  • Developmental Biology

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