Apoptosis-like, reversible changes in plasma membrane asymmetry and permeability, and transient modifications in mitochondrial membrane potential induced by curcumin in rat thymocytes

Ewa Jaruga, Stefano Salvioli, Jurek Dobrucki, Sławomir Chrul, Joanna Bandorowicz-Pikuła, Ewa Sikora, Claudio Franceschi, Andrea Cossarizza, Grzegorz Bartosz

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Curcumin (diferuoylmethane) is a natural compound with anticarcinogenic activities which is able to exert either proapoptotic or antiapoptotic effects in different cell types. This paper focuses on the sequence and extent of primary events induced by curcumin, in comparison with those occurring during dexamethasone-induced apoptosis in rat thymocytes. It also presents annexin VI-FITC as a new probe for studying membrane asymmetry. Curcumin readily penetrates into the cytoplasm, and is able to accumulate in membranous structures such as plasma membrane, endoplasmic reticulum and nuclear envelope. Curcumin-treated cells exhibit typical features of apoptotic cell death, including shrinkage, transient phosphatidylserine exposure, increased membrane permeability and decrease in mitochondrial membrane potential. However, nuclei morphology, DNA fragmentation, the extent and time-course of membrane changes are different from those observed during dexamethasone-induced apoptosis, suggesting that, despite many similarities, the mode of action and the events triggered by curcumin are different from those occurring during typical apoptosis.

Original languageEnglish
Pages (from-to)287-293
Number of pages7
JournalFEBS Letters
Volume433
Issue number3
Publication statusPublished - Aug 21 1998

Fingerprint

Curcumin
Mitochondrial Membrane Potential
Thymocytes
Cell membranes
Rats
Permeability
Cell Membrane
Apoptosis
Membranes
Dexamethasone
Annexin A6
Fluorescein-5-isothiocyanate
Phosphatidylserines
Nuclear Envelope
DNA Fragmentation
Cell death
Endoplasmic Reticulum
Cytoplasm
Cell Death
Cells

Keywords

  • Apoptosis
  • Curcumin
  • Membrane asymmetry
  • Mitochondrial membrane potential
  • Permeability

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Jaruga, E., Salvioli, S., Dobrucki, J., Chrul, S., Bandorowicz-Pikuła, J., Sikora, E., ... Bartosz, G. (1998). Apoptosis-like, reversible changes in plasma membrane asymmetry and permeability, and transient modifications in mitochondrial membrane potential induced by curcumin in rat thymocytes. FEBS Letters, 433(3), 287-293.

Apoptosis-like, reversible changes in plasma membrane asymmetry and permeability, and transient modifications in mitochondrial membrane potential induced by curcumin in rat thymocytes. / Jaruga, Ewa; Salvioli, Stefano; Dobrucki, Jurek; Chrul, Sławomir; Bandorowicz-Pikuła, Joanna; Sikora, Ewa; Franceschi, Claudio; Cossarizza, Andrea; Bartosz, Grzegorz.

In: FEBS Letters, Vol. 433, No. 3, 21.08.1998, p. 287-293.

Research output: Contribution to journalArticle

Jaruga, E, Salvioli, S, Dobrucki, J, Chrul, S, Bandorowicz-Pikuła, J, Sikora, E, Franceschi, C, Cossarizza, A & Bartosz, G 1998, 'Apoptosis-like, reversible changes in plasma membrane asymmetry and permeability, and transient modifications in mitochondrial membrane potential induced by curcumin in rat thymocytes', FEBS Letters, vol. 433, no. 3, pp. 287-293.
Jaruga, Ewa ; Salvioli, Stefano ; Dobrucki, Jurek ; Chrul, Sławomir ; Bandorowicz-Pikuła, Joanna ; Sikora, Ewa ; Franceschi, Claudio ; Cossarizza, Andrea ; Bartosz, Grzegorz. / Apoptosis-like, reversible changes in plasma membrane asymmetry and permeability, and transient modifications in mitochondrial membrane potential induced by curcumin in rat thymocytes. In: FEBS Letters. 1998 ; Vol. 433, No. 3. pp. 287-293.
@article{d118b400e29446789a1252860f25a512,
title = "Apoptosis-like, reversible changes in plasma membrane asymmetry and permeability, and transient modifications in mitochondrial membrane potential induced by curcumin in rat thymocytes",
abstract = "Curcumin (diferuoylmethane) is a natural compound with anticarcinogenic activities which is able to exert either proapoptotic or antiapoptotic effects in different cell types. This paper focuses on the sequence and extent of primary events induced by curcumin, in comparison with those occurring during dexamethasone-induced apoptosis in rat thymocytes. It also presents annexin VI-FITC as a new probe for studying membrane asymmetry. Curcumin readily penetrates into the cytoplasm, and is able to accumulate in membranous structures such as plasma membrane, endoplasmic reticulum and nuclear envelope. Curcumin-treated cells exhibit typical features of apoptotic cell death, including shrinkage, transient phosphatidylserine exposure, increased membrane permeability and decrease in mitochondrial membrane potential. However, nuclei morphology, DNA fragmentation, the extent and time-course of membrane changes are different from those observed during dexamethasone-induced apoptosis, suggesting that, despite many similarities, the mode of action and the events triggered by curcumin are different from those occurring during typical apoptosis.",
keywords = "Apoptosis, Curcumin, Membrane asymmetry, Mitochondrial membrane potential, Permeability",
author = "Ewa Jaruga and Stefano Salvioli and Jurek Dobrucki and Sławomir Chrul and Joanna Bandorowicz-Pikuła and Ewa Sikora and Claudio Franceschi and Andrea Cossarizza and Grzegorz Bartosz",
year = "1998",
month = "8",
day = "21",
language = "English",
volume = "433",
pages = "287--293",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Apoptosis-like, reversible changes in plasma membrane asymmetry and permeability, and transient modifications in mitochondrial membrane potential induced by curcumin in rat thymocytes

AU - Jaruga, Ewa

AU - Salvioli, Stefano

AU - Dobrucki, Jurek

AU - Chrul, Sławomir

AU - Bandorowicz-Pikuła, Joanna

AU - Sikora, Ewa

AU - Franceschi, Claudio

AU - Cossarizza, Andrea

AU - Bartosz, Grzegorz

PY - 1998/8/21

Y1 - 1998/8/21

N2 - Curcumin (diferuoylmethane) is a natural compound with anticarcinogenic activities which is able to exert either proapoptotic or antiapoptotic effects in different cell types. This paper focuses on the sequence and extent of primary events induced by curcumin, in comparison with those occurring during dexamethasone-induced apoptosis in rat thymocytes. It also presents annexin VI-FITC as a new probe for studying membrane asymmetry. Curcumin readily penetrates into the cytoplasm, and is able to accumulate in membranous structures such as plasma membrane, endoplasmic reticulum and nuclear envelope. Curcumin-treated cells exhibit typical features of apoptotic cell death, including shrinkage, transient phosphatidylserine exposure, increased membrane permeability and decrease in mitochondrial membrane potential. However, nuclei morphology, DNA fragmentation, the extent and time-course of membrane changes are different from those observed during dexamethasone-induced apoptosis, suggesting that, despite many similarities, the mode of action and the events triggered by curcumin are different from those occurring during typical apoptosis.

AB - Curcumin (diferuoylmethane) is a natural compound with anticarcinogenic activities which is able to exert either proapoptotic or antiapoptotic effects in different cell types. This paper focuses on the sequence and extent of primary events induced by curcumin, in comparison with those occurring during dexamethasone-induced apoptosis in rat thymocytes. It also presents annexin VI-FITC as a new probe for studying membrane asymmetry. Curcumin readily penetrates into the cytoplasm, and is able to accumulate in membranous structures such as plasma membrane, endoplasmic reticulum and nuclear envelope. Curcumin-treated cells exhibit typical features of apoptotic cell death, including shrinkage, transient phosphatidylserine exposure, increased membrane permeability and decrease in mitochondrial membrane potential. However, nuclei morphology, DNA fragmentation, the extent and time-course of membrane changes are different from those observed during dexamethasone-induced apoptosis, suggesting that, despite many similarities, the mode of action and the events triggered by curcumin are different from those occurring during typical apoptosis.

KW - Apoptosis

KW - Curcumin

KW - Membrane asymmetry

KW - Mitochondrial membrane potential

KW - Permeability

UR - http://www.scopus.com/inward/record.url?scp=0032555342&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032555342&partnerID=8YFLogxK

M3 - Article

C2 - 9744813

AN - SCOPUS:0032555342

VL - 433

SP - 287

EP - 293

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 3

ER -