Apoptosis of human primary osteoclasts treated with molecules targeting nuclear factor-κB

Roberta Piva, Letizia Penolazzi, Monica Borgatti, Ilaria Lampronti, Elisabetta Lambertini, Elena Torreggiani, Roberto Gambari

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Osteoclasts (OCs) are involved in several pathologies associated with bone loss, including rheumatoid arthritis, osteoporosis, bone metastasis of myeloma, osteosarcoma, and breast cancer. In this review we determined the effects of natural compounds, including extracts from medicinal plants, on differentiation and survival of human primary OCs obtained from peripheral blood. We found that OCs from umbilical cord blood and peripheral blood behave differently in response to molecules inducing apoptosis in this experimental system. Apoptosis induced by decoy oligonucleotides was reproducibly obtained in OCs from peripheral blood but not in OCs derived from cord blood. With respect to effects of medicinal plants, we found that crude extracts of Emblica officinalis are able to induce specifically programmed cell death of mature OCs without altering the process of osteoclastogenesis. E. officinalis specifically increased the expression levels of Fas, a critical member of the apoptotic pathway. Gel shift experiments BioPharmaNet demonstrate that E. officinalis extracts specifically compete with the binding of a transcription factor involved in osteoclastogenesis NF-κB to its specific target DNA sequences. This might explain the observed effects of E. officinalis on the expression levels of IL-6, an NF-κB-specific target gene. We suggest the application of natural products as an alternative tool for therapy applied to bone diseases.

Original languageEnglish
Title of host publicationAnnals of the New York Academy of Sciences
Pages448-456
Number of pages9
Volume1171
DOIs
Publication statusPublished - Aug 2009

Publication series

NameAnnals of the New York Academy of Sciences
Volume1171
ISSN (Print)00778923
ISSN (Electronic)17496632

Keywords

  • Apoptosis
  • Emblica officinalis
  • Medicinal plants
  • Osteoclast
  • Osteoporosis
  • Rheumatoid arthritis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Piva, R., Penolazzi, L., Borgatti, M., Lampronti, I., Lambertini, E., Torreggiani, E., & Gambari, R. (2009). Apoptosis of human primary osteoclasts treated with molecules targeting nuclear factor-κB. In Annals of the New York Academy of Sciences (Vol. 1171, pp. 448-456). (Annals of the New York Academy of Sciences; Vol. 1171). https://doi.org/10.1111/j.1749-6632.2009.04906.x