Apoptosis-related mortality in vitro of mononuclear cells from patients with HIV infection correlates with disease severity and progression

F. Pandolfi, M. Pierdominici, A. Oliva, G. D'Offizi, I. Mezzaroma, B. Mollicone, A. Giovannetti, L. Rainaldi, I. Quinti, F. Aiuti

Research output: Contribution to journalArticlepeer-review

Abstract

Peripheral blood mononuclear cells (PBMC) from 103 HIV-infected patients were tested for their mortality rate (MR) when incubated in vitro for 3 days in a culture medium. MR was related to apoptosis as shown by DNA analysis and morphological evaluation of ethidium bromide-stained PBMC by flow cytometry. MR was significantly higher in patients in CDC stage IV as compared to patients in stage II or III (p = 0.017), MR was also higher in patients with low CD4 cells/mm3 (p = 0.014 for patients with 3) and with low percentage of CD4 cells (p = 0.001 for patients with <10% of CD4 cells). A significant negative correlation was observed between MR and both absolute numbers or percentages of CD4 cells (p <0.001). The addition of interleukin-2 (IL-2) and fibroblast-conditioned medium (FCM) to the cultures significantly reduced MR. However, the ability of both IL-2 and FCM to preserve viability was significantly associated with p24 negativity. Clinical and immunological follow-up was available for 60 patients for a mean period of 26 months. MR at the beginning of the study was significantly higher in the group of patients who clinically progressed (according to the CDC classification) or died during the follow-up (p <0.0001). Our data suggest that MR correlates with both disease severity and progression and that MR is directly related to the depletion of CD4 cells in cultures. We also propose that CD4 depletion contributes to increased in vitro cell death observed in HIV infection through impaired cytokine production.

Original languageEnglish
Pages (from-to)450-458
Number of pages9
JournalJournal of Acquired Immune Deficiency Syndromes and Human Retrovirology
Volume9
Issue number5
Publication statusPublished - 1995

Keywords

  • Apoptosis
  • CD4 lymphocytes
  • Fibroblast-derived factors
  • HIV-1 infection
  • IL- 2

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Immunology and Allergy

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