Apoptotic cell clearance in systemic lupus erythematosus: II. Role of β2-glycoprotein I

Angelo A. Manfredi, Patrizia Rovere, Silvia Heltai, Giacomo Galati, Gaia Nebbia, Angela Tincani, Genesio Balestrieri, Maria Grazia Sabbadini

Research output: Contribution to journalArticle

Abstract

Objective. To analyze the contribution of β2-glycoprotein I (β2- GPI) to apoptotic cell recognition by antiphospholipid antibodies (aPL) and macrophages from patients with autoimmune disease. Methods. Phosphatidylserine expression by Jurkat cells undergoing apoptosis upon CD95 crosslinking or ultraviolet irradiation was verified by confocal microscopy of cells stained with fluorescein isothiocyanate-labeled annexin V. β2-GPI was purified by heparin/cationic-exchange chromatography and was biotinylated or used to purify β2-GPI-specific antibodies by affinity chromatography. Binding to apoptotic cells was assessed by flow cytometry. The clearance of 3H-labeled, apoptotic cells by macrophages was assessed by beta counting. Results. The array of epitopes generated by β2-GPI association with apoptotic cells specifically targets their recognition and is required for their opsonization by human aPL. Nevertheless, β2-GPI is not required for apoptotic cell clearance by human macrophages in the absence of aPL. Conclusion. The proinflammatory clearance of aPL-opsonized apoptotic cells, but not the nonphlogistic clearance of apoptotic cells by scavenger macrophages, depends on β2-GPI.

Original languageEnglish
Pages (from-to)215-223
Number of pages9
JournalArthritis and Rheumatism
Volume41
Issue number2
DOIs
Publication statusPublished - Feb 1998

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

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