Apoptotic hepatocytes become insoluble in detergents and chaotropic agents as a result of transglutaminase action

Laszlo Fesus, Vilmos Thomazy, Francesco Autuori, Maria P. Ceru, Edit Tarcsa, Mauro Piacentini

Research output: Contribution to journalArticlepeer-review

Abstract

Physiological deletion of cells ensues programmed death which involves formation of apoptotic bodies with fragmented DNA. Here we report that apoptotic hepatocytes are insoluble in detergents, urea, guanidine hydrochloride, reducing agents and thereby can be isolated from rat liver following collagenase treatment. They are wrinkled, spherical structures similar to cornified envelopes of epidermis by phase-contrast microscopy and show irregular, globular morphology by scanning-electron microscopy. Part of their DNA content is cleaved into nueleosomal and oligonucleosomal fragments. Their insolubility, like that of the cornified envelope, is evoked by ε-(γ-glutamyl)lysine and N1,N8-bis(γ-glutamyl)spermidine protein cross-linking bonds formed by transglutaminase.

Original languageEnglish
Pages (from-to)150-154
Number of pages5
JournalFEBS Letters
Volume245
Issue number1-2
DOIs
Publication statusPublished - Mar 13 1989

Keywords

  • Apoptosis
  • Protein crosslinkage
  • Terminal differentiation
  • Transglutaminase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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