APP mutations in the Aβ coding region are associated with abundant cerebral deposition of Aβ38

Maria Luisa Moro, Giorgio Giaccone, Raffaella Lombardi, Antonio Indaco, Andrea Uggetti, Michela Morbin, Stefania Saccucci, Giuseppe Di Fede, Marcella Catania, Dominic M. Walsh, Andrea Demarchi, Annemieke Rozemuller, Nenad Bogdanovic, Orso Bugiani, Bernardino Ghetti, Fabrizio Tagliavini

Research output: Contribution to journalArticle

Abstract

Aβ is the main component of amyloid deposits in Alzheimer disease (AD) and its aggregation into oligomers, protofibrils and fibrils is considered a seminal event in the pathogenesis of AD. Aβ with C-terminus at residue 42 is the most abundant species in parenchymal deposits, whereas Aβ with C-terminus at residue 40 predominates in the amyloid of the walls of large vessels. Aβ peptides with other C-termini have not yet been thoroughly investigated. We analysed Aβ38 in the brains of patients with Aβ deposition linked to sporadic and familial AD, hereditary cerebral haemorrhage with amyloidosis, or Down syndrome. Immunohistochemistry, confocal microscopy, immunoelectron microscopy, immunoprecipitation and the electrophoresis separation of low molecular weight aggregates revealed that Aβ38 accumulates consistently in the brains of patients carrying APP mutations in the Aβ coding region, but was not detected in the patients with APP mutations outside the Aβ domain, in the patients with presenilin mutations or in subjects with Down syndrome. In the patients with sporadic AD, Aβ38 was absent in the senile plaques, but it was detected only in the vessel walls of a small subset of patients with severe cerebral amyloid angiopathy. Our results suggest that APP mutations in the Aβ coding region favour Aβ38 accumulation in the brain and that the molecular mechanisms of Aβ deposition in these patients may be different from those active in patients with familial AD associated with other genetic defects and sporadic AD.

Original languageEnglish
Pages (from-to)809-821
Number of pages13
JournalActa Neuropathologica
Volume124
Issue number6
DOIs
Publication statusPublished - Dec 2012

Keywords

  • Aβ38
  • Alzheimer's disease
  • Amyloid
  • Familial disease
  • Immunohistochemistry

ASJC Scopus subject areas

  • Clinical Neurology
  • Pathology and Forensic Medicine
  • Cellular and Molecular Neuroscience

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  • Cite this

    Moro, M. L., Giaccone, G., Lombardi, R., Indaco, A., Uggetti, A., Morbin, M., Saccucci, S., Di Fede, G., Catania, M., Walsh, D. M., Demarchi, A., Rozemuller, A., Bogdanovic, N., Bugiani, O., Ghetti, B., & Tagliavini, F. (2012). APP mutations in the Aβ coding region are associated with abundant cerebral deposition of Aβ38. Acta Neuropathologica, 124(6), 809-821. https://doi.org/10.1007/s00401-012-1061-x