Applicability of [11C]PIB micro-PET imaging for in vivo follow-up of anti-amyloid treatment effects in APP23 mouse model

Anniina Snellman, Johanna Rokka, Francisco R. López-Picón, Semi Helin, Francesca Re, Eliisa Löyttyniemi, Rea Pihlaja, Gianluigi Forloni, Mario Salmona, Massimo Masserini, Olof Solin, Juha O. Rinne, Merja Haaparanta-Solin

Research output: Contribution to journalArticle

Abstract

In this study, we evaluated the anti-amyloid effect of functionalized nanoliposomes (mApoE-PA-LIP) in a mouse model of Alzheimer's disease with use of positron emission tomography and β-amyloid (Aβ)–targeted tracer [11C]Pittsburgh compound B ([11C]PIB). APP23 mice were injected with mApoE-PA-LIP or saline (3 times per week for 3 weeks) and [11C]PIB imaging was performed at baseline, after the treatment and after 3 months follow-up period, accompanied by Aβ immunohistochemistry and ELISA. After the treatment, [11C]PIB binding ratios between mApoE-PA-LIP and saline groups were equivalent in all analyzed brain regions; however, in the saline group, binding ratios increased from the baseline, whereas no increase was detected in the mApoE-PA-LIP group. During the additional follow-up, [11C]PIB binding increased significantly from baseline in both groups, and binding ratios correlated with the immunohistochemically defined Aβ load. This study further supports the use of [11C]PIB positron emission tomography imaging as a biomarker of Aβ deposition in APP23 mice and highlights the benefits of noninvasive follow-up, that is, using baseline data for animal stratification and normalization of treatment effects to baseline values, for future anti-amyloid treatment studies.

Original languageEnglish
Pages (from-to)84-94
Number of pages11
JournalNeurobiology of Aging
Volume57
DOIs
Publication statusPublished - Sep 1 2017

Keywords

  • Alzheimer's disease
  • APP23
  • Liposomes
  • PET
  • [C]PIB
  • β-amyloid

ASJC Scopus subject areas

  • Neuroscience(all)
  • Ageing
  • Clinical Neurology
  • Developmental Biology
  • Geriatrics and Gerontology

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    Snellman, A., Rokka, J., López-Picón, F. R., Helin, S., Re, F., Löyttyniemi, E., Pihlaja, R., Forloni, G., Salmona, M., Masserini, M., Solin, O., Rinne, J. O., & Haaparanta-Solin, M. (2017). Applicability of [11C]PIB micro-PET imaging for in vivo follow-up of anti-amyloid treatment effects in APP23 mouse model. Neurobiology of Aging, 57, 84-94. https://doi.org/10.1016/j.neurobiolaging.2017.05.008