TY - JOUR
T1 - Application of capillary electrophoresis at low pH to oligonucleotides quality control
AU - Pietta, Pier Giorgio
AU - Mangano, Maria Flora
AU - Battaglia, Cristina
AU - Salani, Giuliana
AU - Bernardi, Luigi Rossi
AU - De Bellis, Gianluca
PY - 1999/8/20
Y1 - 1999/8/20
N2 - A method for oligonucleotides analysis by using capillary electrophoresis at low pH in free solution is described. It may be considered an alternative to classical analytical techniques which use basic buffers and require the presence of sieving media to separate oligonucleotides as a function of their length. On the contrary, at low pH oligo nucleotides can be separated only depending on their base composition. A large set of samples consisting of 72 synthetic oligonucleotides bearing a 5'-alkylamine moiety and designed for HLA genotyping were analysed. The quality of these synthetic oligos was easily assessed, and a single base difference in oligonucleotides of equal sequence was detected. The results suggest the application of this method to the emerging field of mutation detection and single nucleotide polymorfism analysis. Copyright (C) 1999 Elsevier Science B.V.
AB - A method for oligonucleotides analysis by using capillary electrophoresis at low pH in free solution is described. It may be considered an alternative to classical analytical techniques which use basic buffers and require the presence of sieving media to separate oligonucleotides as a function of their length. On the contrary, at low pH oligo nucleotides can be separated only depending on their base composition. A large set of samples consisting of 72 synthetic oligonucleotides bearing a 5'-alkylamine moiety and designed for HLA genotyping were analysed. The quality of these synthetic oligos was easily assessed, and a single base difference in oligonucleotides of equal sequence was detected. The results suggest the application of this method to the emerging field of mutation detection and single nucleotide polymorfism analysis. Copyright (C) 1999 Elsevier Science B.V.
KW - Oligonucleotides
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U2 - 10.1016/S0021-9673(99)00451-3
DO - 10.1016/S0021-9673(99)00451-3
M3 - Article
C2 - 10486742
AN - SCOPUS:0032790394
VL - 853
SP - 355
EP - 358
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 1-2
ER -