Application of capillary electrophoresis at low pH to oligonucleotides quality control

Pier Giorgio Pietta, Maria Flora Mangano, Cristina Battaglia, Giuliana Salani, Luigi Rossi Bernardi, Gianluca De Bellis

Research output: Contribution to journalArticlepeer-review

Abstract

A method for oligonucleotides analysis by using capillary electrophoresis at low pH in free solution is described. It may be considered an alternative to classical analytical techniques which use basic buffers and require the presence of sieving media to separate oligonucleotides as a function of their length. On the contrary, at low pH oligo nucleotides can be separated only depending on their base composition. A large set of samples consisting of 72 synthetic oligonucleotides bearing a 5'-alkylamine moiety and designed for HLA genotyping were analysed. The quality of these synthetic oligos was easily assessed, and a single base difference in oligonucleotides of equal sequence was detected. The results suggest the application of this method to the emerging field of mutation detection and single nucleotide polymorfism analysis. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)355-358
Number of pages4
JournalJournal of Chromatography A
Volume853
Issue number1-2
DOIs
Publication statusPublished - Aug 20 1999

Keywords

  • Oligonucleotides

ASJC Scopus subject areas

  • Analytical Chemistry

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